20.109(S08): Spring 2008 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 5	ANS		Orientation	Slides are here (warning: .ppt download)
1	1	Thur Feb 7	BE		Day 1: DNA engineering using PCR	PDF of slides is here
1	2	Tue Feb 12	BE		Day 2: Clean and cut DNA	PDF of slides is here
1	3	Thur Feb 14	BE		Day 3: Agarose gel electrophoresis
		President's Day Feb 18				No lab Feb 19 or Feb 20
1	4	Thur Feb 21	BE		Day 4: DNA ligation and bacterial transformation
1	5	Tue Feb 26	BE		Day 5: Examine candidate clones
1	6	Thur Feb 28	BE		Day 6: Restriction map and tissue culture
1	7	Tue Mar 4	BE		Day 7: Lipofection
1	8	Thur Mar 6	BE		Day 8: FACS analysis
2	1	Tue Mar 11	AJ		Day 1: Start-up protein engineering
2	2	Thur Mar 13	AJ		Day 2: Site-directed mutagenesis
2	3	Tue Mar 18	AJ		Day 3: Prepare expression system
2	4	Thur Mar 20	AJ		Day 4: Induce protein expression
		Spring Break Mar 24-28				No lab all week
2	5	Tue Apr 1	AJ		Day 5: Characterize protein expression
2	6	Thur Apr 3	AJ		Day 6: Assay protein behavior
2	7	Tue Apr 8	AJ		Day 7: Data analysis
2	8	Thur Apr 10	AJ		Day 8: Student presentations
3	1	Tues Apr 15	ANS		Day 1: Start-up biomaterials engineering
3	2	Thur Apr 17	ANS		Day 2: Initiate cell culture
		Patriot's Day April 21-22				No lab Apr 22 or Apr 23
3	3	Thur Apr 24	ANS		Module 3 Day 3: Testing cell viability
3	4	Tue Apr 29	ANS		Module 3 Day 4: Preparing cells for analysis
3	5	Thur May 1	ANS		Module 3 Day 5: Transcript-level analysis
3	6	Tue May 6	ANS		Module 3 Day 6: Protein-level analysis
3	7	Thur May 8	ANS		Module 3 Day 7: Wrap-up analysis
3	8	Tue May 13	ANS		Module 3 Day 8: Student presentations
		Thur May 15			No lab session. Evaluations during lecture, then celebratory lunch, place TBD.