20.109(S08): Spring 2008 schedule: Difference between revisions

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| Tue Feb 5  
| Tue Feb 5  
| ANS
| ANS
|  
| VL, BE
| [[20.109(S08):Lab tour | Orientation]]
| [[20.109(S08):Lab tour | Orientation]]
| Slides are [[Media:20.109_OrientationLect(S08).ppt|here]] (warning: .ppt download)
| Slides are [[Media:20.109_OrientationLect(S08).ppt|here]] (warning: .ppt download)
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| Thur Feb 7
| Thur Feb 7
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]]
| [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]]
| PDF of slides is [[Media:BE109_S08_Mod1-Day1_ENGELWARD.pdf|here]]
| PDF of slides is [[Media:BE109_S08_Mod1-Day1_ENGELWARD.pdf|here]]
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| Tue Feb 12  
| Tue Feb 12  
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
| PDF of slides is [[Media:BE109_S08_Mod1-Day2_ENGELWARD.pdf|here]]
| PDF of slides is [[Media:BE109_S08_Mod1-Day2_ENGELWARD.pdf|here]]
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| Thur Feb 14
| Thur Feb 14
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]]
| [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]]
|
|
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| Thur Feb 21
| Thur Feb 21
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]]
| [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]]
|  
|  
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| Tue Feb 26
| Tue Feb 26
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]]
| [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]]
|  
|  
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| Thur Feb 28
| Thur Feb 28
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]]
| [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]]
|  
|  
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| Tue Mar 4  
| Tue Mar 4  
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]]
| [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]]
|
|
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| Thur Mar 6
| Thur Mar 6
| BE
| BE
|  
| DW
| [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]]
| [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]]
|
|

Revision as of 21:00, 12 February 2008


20.109(S08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 5 ANS VL, BE Orientation Slides are here (warning: .ppt download)
1 1 Thur Feb 7 BE DW Day 1: DNA engineering using PCR PDF of slides is here
1 2 Tue Feb 12 BE DW Day 2: Clean and cut DNA PDF of slides is here
1 3 Thur Feb 14 BE DW Day 3: Agarose gel electrophoresis
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur Feb 21 BE DW Day 4: DNA ligation and bacterial transformation
1 5 Tue Feb 26 BE DW Day 5: Examine candidate clones
1 6 Thur Feb 28 BE DW Day 6: Restriction map and tissue culture
1 7 Tue Mar 4 BE DW Day 7: Lipofection
1 8 Thur Mar 6 BE DW Day 8: FACS analysis
2 1 Tue Mar 11 AJ Day 1: Start-up protein engineering
2 2 Thur Mar 13 AJ Day 2: Site-directed mutagenesis
2 3 Tue Mar 18 AJ Day 3: Prepare expression system
2 4 Thur Mar 20 AJ Day 4: Induce protein expression
Spring Break Mar 24-28 No lab all week
2 5 Tue Apr 1 AJ Day 5: Characterize protein expression
2 6 Thur Apr 3 AJ Day 6: Assay protein behavior
2 7 Tue Apr 8 AJ Day 7: Data analysis
2 8 Thur Apr 10 AJ Day 8: Student presentations
3 1 Tues Apr 15 ANS Day 1: Start-up biomaterials engineering
3 2 Thur Apr 17 ANS Day 2: Initiate cell culture
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 3 Thur Apr 24 ANS Module 3 Day 3: Testing cell viability
3 4 Tue Apr 29 ANS Module 3 Day 4: Preparing cells for analysis
3 5 Thur May 1 ANS Module 3 Day 5: Transcript-level analysis
3 6 Tue May 6 ANS Module 3 Day 6: Protein-level analysis
3 7 Thur May 8 ANS Module 3 Day 7: Wrap-up analysis
3 8 Tue May 13 ANS Module 3 Day 8: Student presentations
Thur May 15 No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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