20.109(S08): Spring 2008 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 5	ANS	VL, BE	Orientation	Slides are here (warning: .ppt download)
1	1	Thur Feb 7	BE	DW	Day 1: DNA engineering using PCR	PDF of slides is here
1	2	Tue Feb 12	BE	DW	Day 2: Clean and cut DNA	PDF of slides is here
1	3	Thur Feb 14	BE	DW	Day 3: Agarose gel electrophoresis	PDF of slides is here
		President's Day Feb 18				No lab Feb 19 or Feb 20
1	4	Thur Feb 21	BE	DW	Day 4: DNA ligation and bacterial transformation	PDF of slides is here
1	5	Tue Feb 26	BE	DW	Day 5: Examine candidate clones	PDF of slides is here
1	6	Thur Feb 28	BE	DW	Day 6: Restriction map and tissue culture	PDF of slides is here
1	7	Tue Mar 4	BE	DW	Day 7: Lipofection	PDF of slides is here
1	8	Thur Mar 6	BE	DW	Day 8: FACS analysis	PDF of slides is here
2	1	Tue Mar 11	AJ	VL	Day 1: Start-up protein engineering	'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.)
2	2	Thur Mar 13	AJ	VL	Day 2: Site-directed mutagenesis	For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary.
2	3	Tue Mar 18	AJ	VL	Day 3: Prepare expression system	For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary.
2	4	Thur Mar 20	AJ	VL	Day 4: Induce protein expression	For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary.
		Spring Break Mar 24-28				No lab all week
2	5	Tue Apr 1	AJ	VL	Day 5: Characterize protein expression	For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary.
2	6	Thur Apr 3	AJ	VL	Day 6: Assay protein behavior	Please see Attisa's user page
2	7	Tue Apr 8	AJ	VL	Day 7: Data analysis	'High throughput screening' handout is available in class.
2	8	Thur Apr 10	AJ	VL	Day 8: Student presentations	'"Real-world" protein engineering' handout is available in class.
3	1	Tues Apr 15	ANS	BE	Day 1: Start-up biomaterials engineering
3	2	Thur Apr 17	ANS	BE	Day 2: Initiate cell culture
		Patriot's Day April 21-22				No lab Apr 22 or Apr 23
3	3	Thur Apr 24	ANS	BE	Module 3 Day 3: Testing cell viability
3	4	Tue Apr 29	ANS	BE	Module 3 Day 4: Preparing cells for analysis
3	5	Thur May 1	ANS	BE	Module 3 Day 5: Transcript-level analysis
3	6	Tue May 6	ANS	BE	Module 3 Day 6: Protein-level analysis
3	7	Thur May 8	ANS	BE	Module 3 Day 7: Wrap-up analysis
3	8	Tue May 13	ANS	BE	Module 3 Day 8: Student presentations
		Thur May 15			No lab session. Evaluations during lecture, then celebratory lunch, place TBD.