20.109(S08): Spring 2008 schedule
From OpenWetWare
| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 5 | ANS | VL, BE | Orientation | Slides are here (warning: .ppt download) | ||
| 1 | 1 | Thur Feb 7 | BE | DW | Day 1: DNA engineering using PCR | PDF of slides is here |
| 1 | 2 | Tue Feb 12 | BE | DW | Day 2: Clean and cut DNA | PDF of slides is here |
| 1 | 3 | Thur Feb 14 | BE | DW | Day 3: Agarose gel electrophoresis | PDF of slides is here |
| President's Day Feb 18 | No lab Feb 19 or Feb 20 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 21 | BE | DW | Day 4: DNA ligation and bacterial transformation | PDF of slides is here |
| 1 | 5 | Tue Feb 26 | BE | DW | Day 5: Examine candidate clones | PDF of slides is here |
| 1 | 6 | Thur Feb 28 | BE | DW | Day 6: Restriction map and tissue culture | PDF of slides is here |
| 1 | 7 | Tue Mar 4 | BE | DW | Day 7: Lipofection | PDF of slides is here |
| 1 | 8 | Thur Mar 6 | BE | DW | Day 8: FACS analysis | PDF of slides is here |
| 2 | 1 | Tue Mar 11 | AJ | VL | Day 1: Start-up protein engineering | 'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.) |
| 2 | 2 | Thur Mar 13 | AJ | VL | Day 2: Site-directed mutagenesis | For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary. |
| 2 | 3 | Tue Mar 18 | AJ | VL | Day 3: Prepare expression system | For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary. |
| 2 | 4 | Thur Mar 20 | AJ | VL | Day 4: Induce protein expression | For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary. |
| Spring Break Mar 24-28 | No lab all week | |||||
| 2 | 5 | Tue Apr 1 | AJ | VL | Day 5: Characterize protein expression | For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary. |
| 2 | 6 | Thur Apr 3 | AJ | VL | Day 6: Assay protein behavior | Please see Attisa's user page |
| 2 | 7 | Tue Apr 8 | AJ | VL | Day 7: Data analysis | 'High throughput screening' handout is available in class. |
| 2 | 8 | Thur Apr 10 | AJ | VL | Day 8: Student presentations |
'"Real-world" protein engineering' handout is available in class. |
| 3 | 1 | Tues Apr 15 | ANS | BE | Day 1: Start-up biomaterials engineering | PDF of slides is here |
| 3 | 2 | Thur Apr 17 | ANS | BE | Day 2: Initiate cell culture | |
| Patriot's Day April 21-22 | No lab Apr 22 or Apr 23 | |||||
| 3 | 3 | Thur Apr 24 | ANS | BE | Module 3 Day 3: Testing cell viability | |
| 3 | 4 | Tue Apr 29 | ANS | BE | Module 3 Day 4: Preparing cells for analysis | |
| 3 | 5 | Thur May 1 | ANS | BE | Module 3 Day 5: Transcript-level analysis | |
| 3 | 6 | Tue May 6 | ANS | BE | Module 3 Day 6: Protein-level analysis | |
| 3 | 7 | Thur May 8 | ANS | BE | Module 3 Day 7: Wrap-up analysis | |
| 3 | 8 | Tue May 13 | ANS | BE | Module 3 Day 8: Student presentations | |
| Thur May 15 | No lab session. Evaluations during lecture, then celebratory lunch, place TBD. | |||||
