20.109(S09)/PinkWF: Difference between revisions
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*http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html | *http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html | ||
**Proteins bind to promoter regulatory sites to repress or activate expression. This results in two stable states: proteins (repressor) repress their own repressor, so they will remain stably expressed. But, switching is controllable switching: dominant repressor (state) can be de-activated with addition of inducer (IPTG, thermal shock, etc). This allows expression of the other repressor, and enables controllable state change of the system | **Proteins bind to promoter regulatory sites to repress or activate expression. This results in two stable states: proteins (repressor) repress their own repressor, so they will remain stably expressed. But, switching is controllable switching: dominant repressor (state) can be de-activated with addition of inducer (IPTG, thermal shock, etc). This allows expression of the other repressor, and enables controllable state change of the system | ||
* http://www.ncbi.nlm.nih.gov/pubmed/3289117?dopt=Abstract | |||
** Leucine zippers are a common structures that have key functions within binding proteins. | |||
Revision as of 10:28, 6 May 2009
20.109 Research Proposal for Derek Ju and Alvin Chen
Project Overview
Our goal is to rewire a bacterium to "miniprep" itself, when given some sort of an external stimuli (such as sensing of dense cell growth). This project will serve the following purposes:
- Make the research process easier, cheaper, and more efficient for scientists working with bacteria.
- Most laboratory strains have been genetically modified for scientific use; however our bacteria would be the first to be modified to do an experiment on itself.
- Self-lysing of cells when dense cell growth is encountered - less chance for environmental contamination if genetically engineered bacteria escape from the lab
- Serve as an example of a way synthetic biology can be used for cellular control
- Be an application of using irreversible switches within genetic circuits
- Be an example of sensors being built into bacteria
Background
Research Problems and Goals
Project Details and Methods
Predicted Outcomes
Resources Needed
References
- http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html
- Proteins bind to promoter regulatory sites to repress or activate expression. This results in two stable states: proteins (repressor) repress their own repressor, so they will remain stably expressed. But, switching is controllable switching: dominant repressor (state) can be de-activated with addition of inducer (IPTG, thermal shock, etc). This allows expression of the other repressor, and enables controllable state change of the system
- http://www.ncbi.nlm.nih.gov/pubmed/3289117?dopt=Abstract
- Leucine zippers are a common structures that have key functions within binding proteins.
