20.109(S10): Spring 2010 schedule: Difference between revisions
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| 1 | | 1 | ||
| Thur Feb 4 | | Thur Feb 4 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]] | | [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]] | ||
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| 2 | | 2 | ||
| Tue Feb 9 | | Tue Feb 9 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]] | | [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]] | ||
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| 3 | | 3 | ||
| Thur Feb 11 | | Thur Feb 11 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]] | | [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]] | ||
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| 4 | | 4 | ||
| Thur Feb 18 | | Thur Feb 18 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]] | | [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]] | ||
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| 5 | | 5 | ||
| Tue Feb 23 | | Tue Feb 23 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]] | | [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]] | ||
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| 6 | | 6 | ||
| Thur Feb 25 | | Thur Feb 25 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]] | | [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]] | ||
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| 7 | | 7 | ||
| Tue Mar 2 | | Tue Mar 2 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]] | | [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]] | ||
| Line 86: | Line 86: | ||
| 8 | | 8 | ||
| Thur Mar 4 | | Thur Mar 4 | ||
| | | [http://web.mit.edu/be/people/niles.htm JN] | ||
| [[User:Christina_M_Birch| CB]] | | [[User:Christina_M_Birch| CB]] | ||
| [[20.109(S10):Journal club (Day8)| Journal club]] | | [[20.109(S10):Journal club (Day8)| Journal club]] | ||
Revision as of 08:01, 1 February 2010
_frontpg.JPG)
| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 2 | ANS | Orientation | ||||
| 1 | 1 | Thur Feb 4 | JN | CB | Amplify aptamer-encoding DNA | |
| 1 | 2 | Tue Feb 9 | JN | CB | Purify aptamer-encoding DNA | |
| 1 | 3 | Thur Feb 11 | JN | CB | Prepare RNA by IVT | |
| President's Day Feb 15 | No lab Feb 16 or Feb 17 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 18 | JN | CB | Purify RNA and run affinity column | |
| 1 | 5 | Tue Feb 23 | JN | CB | RNA to DNA by RT-PCR | |
| 1 | 6 | Thur Feb 25 | JN | CB | Post-selection IVT and journal club | |
| 1 | 7 | Tue Mar 2 | JN | CB | Aptamer binding assay | |
| 1 | 8 | Thur Mar 4 | JN | CB | Journal club | |
| 2 | 1 | Tue Mar 9 | AJ | XY | Start-up protein engineering | |
| 2 | 2 | Thur Mar 11 | AJ | XY | Site-directed mutagenesis | |
| 2 | 3 | Tue Mar 16 | AJ | XY | Bacterial amplification of DNA | |
| 2 | 4 | Thur Mar 18 | AJ | XY | Prepare expression system | |
| Spring Break Mar 22-26 | No lab all week | |||||
| 2 | 5 | Tue Mar 30 | AJ | XY | Induce protein and evaluate DNA | |
| 2 | 6 | Thur Apr 1 | AJ | XY | Characterize protein expression | |
| 2 | 7 | Tue Apr 6 | AJ | XY | Assay protein behavior | |
| 2 | 8 | Thur Apr 8 | AJ | XY | Data analysis | |
| 3 | 1 | Tues Apr 13 | ANS | |||
| 3 | 2 | Thur Apr 15 | ANS | |||
| Patriot's Day April 19-20 | No lab Apr 20 or Apr 21 | |||||
| 3 | 3 | Thur Apr 22 | ANS | |||
| 3 | 4 | Tue Apr 27 | ANS | |||
| 3 | 5 | Thur Apr 29 | ANS | |||
| 3 | 6 | Tue May 4 | ANS | |||
| 3 | 7 | Thur May 6 | ANS | |||
| 3 | 8 | Tue May 11 | ANS | |||
| Thur May 13 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
