20.109(S10): Spring 2010 schedule

20.109(S10): Laboratory Fundamentals of Biological Engineering

Home People Schedule Spring 2010 Assignments Lab Basics OWW Basics
RNA Engineering Protein Engineering Cell-Biomaterial Engineering

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 2	ANS		Orientation

1	1	Thur Feb 4	JN	CB	Amplify aptamer-encoding DNA
1	2	Tue Feb 9	JN	CB	Purify aptamer-encoding DNA
1	3	Thur Feb 11	JN	CB	Prepare RNA by IVT
		President's Day Feb 15				No lab Feb 16 or Feb 17
1	4	Thur Feb 18	JN	CB	Purify RNA and run affinity column
1	5	Tue Feb 23	JN	CB	RNA to DNA by RT-PCR
1	6	Thur Feb 25	JN	CB	Post-selection IVT and journal club
1	7	Tue Mar 2	JN	CB	Aptamer binding assay
1	8	Thur Mar 4	JN	CB	Journal club

2	1	Tue Mar 9	AJ	XY	Start-up protein engineering
2	2	Thur Mar 11	AJ	XY	Site-directed mutagenesis
2	3	Tue Mar 16	AJ	XY	Bacterial amplification of DNA
2	4	Thur Mar 18	AJ	XY	Prepare expression system
		Spring Break Mar 22-26				No lab all week
2	5	Tue Mar 30	AJ	XY	Induce protein and evaluate DNA
2	6	Thur Apr 1	AJ	XY	Characterize protein expression
2	7	Tue Apr 6	AJ	XY	Assay protein behavior
2	8	Thur Apr 8	AJ	XY	Data analysis

3	1	Tues Apr 13	ANS
3	2	Thur Apr 15	ANS
		Patriot's Day April 19-20				No lab Apr 20 or Apr 21
3	3	Thur Apr 22	ANS
3	4	Tue Apr 27	ANS
3	5	Thur Apr 29	ANS
3	6	Tue May 4	ANS
3	7	Thur May 6	ANS
3	8	Tue May 11	ANS
		Thur May 13			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD

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