20.109(S11): Spring 2011 schedule: Difference between revisions
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| Thur Feb 3 | | Thur Feb 3 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | |[[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]] | | [[20.109(S11):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]] | ||
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| Tue Feb 8 | | Tue Feb 8 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]] | | [[20.109(S11):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]] | ||
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| Line 42: | Line 42: | ||
| Thur Feb 10 | | Thur Feb 10 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]] | | [[20.109(S11):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]] | ||
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| Line 50: | Line 50: | ||
| Tue Feb 15 | | Tue Feb 15 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]] | | [[20.109(S11):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]] | ||
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| Thur Feb 17 | | Thur Feb 17 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]] | | [[20.109(S11):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]] | ||
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| Thur Feb 24 | | Thur Feb 24 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | |[[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]] | | [[20.109(S11):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]] | ||
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| Line 79: | Line 79: | ||
| Tue Mar 1 | | Tue Mar 1 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Aptamer binding assay (Day7)| Aptamer binding assay]] | | [[20.109(S11):Aptamer binding assay (Day7)| Aptamer binding assay]] | ||
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| Line 87: | Line 87: | ||
| Thur Mar 3 | | Thur Mar 3 | ||
| [http://web.mit.edu/be/people/niles.shtml JN] | | [http://web.mit.edu/be/people/niles.shtml JN] | ||
| | | [[User:Carrie M Thompson| CT]] | ||
| [[20.109(S11):Journal club (Day8)| Journal club]] | | [[20.109(S11):Journal club (Day8)| Journal club]] | ||
| | | | ||
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| Tue Mar 8 | | Tue Mar 8 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Explore model system (Day1)| Explore model system]] | |[[20.109(S11):Explore model system (Day1)| Explore model system]] | ||
|<font color = CC0000><b>Module 1 Draft Due</b></font color><br> | |<font color = CC0000><b>Module 1 Draft Due</b></font color><br> | ||
| Line 105: | Line 105: | ||
| Thur Mar 10 | | Thur Mar 10 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Complete DNA design (Day2)| Complete DNA design]] | |[[20.109(S11):Complete DNA design (Day2)| Complete DNA design]] | ||
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| Tue Mar 15 | | Tue Mar 15 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Prepare DNA for cloning (Day3)| Prepare DNA for cloning]] | |[[20.109(S11):Prepare DNA for cloning (Day3)| Prepare DNA for cloning]] | ||
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| Thur Mar 17 | | Thur Mar 17 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Ligate DNA and transform bacteria (Day4)| Ligate DNA and transform bacteria]] | |[[20.109(S11):Ligate DNA and transform bacteria (Day4)| Ligate DNA and transform bacteria]] | ||
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| Line 134: | Line 134: | ||
| Tue Mar 29 | | Tue Mar 29 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Prepare candidate clones in model cell strain (Day5)| Prepare candidate clones in model cell strain]] | |[[20.109(S11):Prepare candidate clones in model cell strain (Day5)| Prepare candidate clones in model cell strain]] | ||
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| Thur Mar 31 | | Thur Mar 31 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Evaluate DNA and choose clone (Day6)| Evaluate DNA and choose clone]] | |[[20.109(S11):Evaluate DNA and choose clone (Day6)| Evaluate DNA and choose clone]] | ||
|<font color = CC0000><b>Module 1 Final Report Due</b></font color><br> | |<font color = CC0000><b>Module 1 Final Report Due</b></font color><br> | ||
| Line 150: | Line 150: | ||
| Tue Apr 5 | | Tue Apr 5 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Assay modified model system (Day7)| Assay modified model system]] | |[[20.109(S11):Assay modified model system (Day7)| Assay modified model system]] | ||
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| Thur Apr 7 | | Thur Apr 7 | ||
| [http://web.mit.edu/be/people/weiss.shtml RW] | | [http://web.mit.edu/be/people/weiss.shtml RW] | ||
| | | [[User:Adrian_Randall| AR]] | ||
|[[20.109(S11):Wrap-up analysis and modelingb (Day8)| Wrap-up analysis and modeling]] | |[[20.109(S11):Wrap-up analysis and modelingb (Day8)| Wrap-up analysis and modeling]] | ||
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| Line 168: | Line 168: | ||
| Tues Apr 12 | | Tues Apr 12 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]] | | [[20.109(S11):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]] | ||
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br> | | <font color = CC0000><b>Module 2 Draft Due</b></font color><br> | ||
| Line 176: | Line 176: | ||
| Thur Apr 14 | | Thur Apr 14 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung|HHH]] | ||
| [[20.109(S11):Initiate cell culture (Day2) | Initiate cell culture]] | | [[20.109(S11):Initiate cell culture (Day2) | Initiate cell culture]] | ||
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| Line 189: | Line 189: | ||
| Thur Apr 21 | | Thur Apr 21 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Testing cell viability (Day3) | Testing cell viability]] | | [[20.109(S11):Testing cell viability (Day3) | Testing cell viability]] | ||
| | | | ||
| Line 197: | Line 197: | ||
| Tue Apr 26 | | Tue Apr 26 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Preparing cells for analysis (Day4) | Preparing cells for analysis]] | | [[20.109(S11):Preparing cells for analysis (Day4) | Preparing cells for analysis]] | ||
| | | | ||
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| Thur Apr 28 | | Thur Apr 28 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]] | | [[20.109(S11):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]] | ||
|<font color = CC0000><b>Module 2 Final Report Due</b></font color><br> | |<font color = CC0000><b>Module 2 Final Report Due</b></font color><br> | ||
| Line 213: | Line 213: | ||
| Tue May 3 | | Tue May 3 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | |[[User:Han-Hwa Hung| HHH]]] | ||
| [[20.109(S11):Transcript-level analysis (Day6)| Transcript-level analysis]] | | [[20.109(S11):Transcript-level analysis (Day6)| Transcript-level analysis]] | ||
| | | | ||
| Line 221: | Line 221: | ||
| Thur May 5 | | Thur May 5 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]] | | [[20.109(S11):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]] | ||
| | | | ||
| Line 229: | Line 229: | ||
| Tue May 10 | | Tue May 10 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | [[User:Han-Hwa Hung| HHH]] | ||
| [[20.109(S11):Student presentations (Day8) | Student presentations]] | | [[20.109(S11):Student presentations (Day8) | Student presentations]] | ||
| | | | ||
Revision as of 18:49, 30 January 2011
_frontpg.JPG)
| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 1 | ANS | Orientation | ||||
| 1 | 1 | Thur Feb 3 | JN | CT | Amplify aptamer-encoding DNA | |
| 1 | 2 | Tue Feb 8 | JN | CT | Purify aptamer-encoding DNA | |
| 1 | 3 | Thur Feb 10 | JN | CT | Prepare RNA by IVT | |
| 1 | 4 | Tue Feb 15 | JN | CT | Purify RNA and run affinity column | |
| 1 | 5 | Thur Feb 17 | JN | CT | RNA to DNA by RT-PCR | |
| President's Day Feb 21 | No lab Feb 22 or Feb 23 | |||||
|---|---|---|---|---|---|---|
| 1 | 6 | Thur Feb 24 | JN | CT | Post-selection IVT and journal club | |
| 1 | 7 | Tue Mar 1 | JN | CT | Aptamer binding assay | |
| 1 | 8 | Thur Mar 3 | JN | CT | Journal club | |
| 2 | 1 | Tue Mar 8 | RW | AR | Explore model system | Module 1 Draft Due |
| 2 | 2 | Thur Mar 10 | RW | AR | Complete DNA design | |
| 2 | 3 | Tue Mar 15 | RW | AR | Prepare DNA for cloning | |
| 2 | 4 | Thur Mar 17 | RW | AR | Ligate DNA and transform bacteria | |
| Spring Break Mar 21-25 | No lab all week | |||||
| 2 | 5 | Tue Mar 29 | RW | AR | Prepare candidate clones in model cell strain | |
| 2 | 6 | Thur Mar 31 | RW | AR | Evaluate DNA and choose clone | Module 1 Final Report Due |
| 2 | 7 | Tue Apr 5 | RW | AR | Assay modified model system | |
| 2 | 8 | Thur Apr 7 | RW | AR | Wrap-up analysis and modeling | |
| 3 | 1 | Tues Apr 12 | ANS | HHH | Start-up biomaterials engineering | Module 2 Draft Due |
| 3 | 2 | Thur Apr 14 | ANS | HHH | Initiate cell culture | |
| Patriot's Day April 18-19 | No lab Apr 19 or Apr 20 | |||||
| 3 | 3 | Thur Apr 21 | ANS | HHH | Testing cell viability | |
| 3 | 4 | Tue Apr 26 | ANS | HHH | Preparing cells for analysis | |
| 3 | 5 | Thur Apr 28 | ANS | HHH | Initiating transcript and protein assays | Module 2 Final Report Due |
| 3 | 6 | Tue May 3 | ANS | HHH] | Transcript-level analysis | |
| 3 | 7 | Thur May 5 | ANS | HHH | Protein-level and wrap-up analysis | |
| 3 | 8 | Tue May 10 | ANS | HHH | Student presentations | |
| Thur May 12 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
