MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
note
|
|
|
Tue Feb 1
|
ANS
|
|
Orientation
|
* Introductory Lecture (PDF) * Intro Pre-Lab, T/R
|
|
1
|
1
|
Thur Feb 3
|
JN
|
CT
|
Amplify aptamer-encoding DNA
|
* M1D1 Lecture * M1D1 Pre-Lab
|
1
|
2
|
Tue Feb 8
|
JN
|
CT
|
Purify aptamer-encoding DNA
|
* M1D2 Lecture * M1D2 Pre-Lab
|
1
|
3
|
Thur Feb 10
|
JN
|
CT
|
Prepare RNA by IVT
|
|
1
|
4
|
Tue Feb 15
|
JN
|
CT
|
Purify RNA and run affinity column
|
|
1
|
5
|
Thur Feb 17
|
JN
|
CT
|
RNA to DNA by RT-PCR
|
|
|
|
President's Day Feb 21
|
No lab Feb 22 or Feb 23
|
1
|
6
|
Thur Feb 24
|
JN
|
CT
|
Post-selection IVT and journal club
|
|
1
|
7
|
Tue Mar 1
|
JN
|
CT
|
Aptamer binding assay
|
|
1
|
8
|
Thur Mar 3
|
JN
|
CT
|
Journal club
|
|
|
2
|
1
|
Tue Mar 8
|
RW
|
AR
|
Explore model system
|
Module 1 Draft Due
|
2
|
2
|
Thur Mar 10
|
RW
|
AR
|
Complete DNA design
|
|
2
|
3
|
Tue Mar 15
|
RW
|
AR
|
Prepare DNA for cloning
|
|
2
|
4
|
Thur Mar 17
|
RW
|
AR
|
Ligate DNA and transform bacteria
|
|
|
|
Spring Break Mar 21-25
|
No lab all week
|
2
|
5
|
Tue Mar 29
|
RW
|
AR
|
Prepare candidate clones in model cell strain
|
|
2
|
6
|
Thur Mar 31
|
RW
|
AR
|
Evaluate DNA and choose clone
|
Module 1 Final Report Due
|
2
|
7
|
Tue Apr 5
|
RW
|
AR
|
Assay modified model system
|
|
2
|
8
|
Thur Apr 7
|
RW
|
AR
|
Wrap-up analysis and modeling
|
|
|
3
|
1
|
Tues Apr 12
|
ANS
|
HHH
|
Start-up biomaterials engineering
|
Module 2 Draft Due
|
3
|
2
|
Thur Apr 14
|
ANS
|
HHH
|
Initiate cell culture
|
|
|
|
Patriot's Day April 18-19
|
No lab Apr 19 or Apr 20
|
3
|
3
|
Thur Apr 21
|
ANS
|
HHH
|
Testing cell viability
|
|
3
|
4
|
Tue Apr 26
|
ANS
|
HHH
|
Preparing cells for analysis
|
|
3
|
5
|
Thur Apr 28
|
ANS
|
HHH
|
Initiating transcript and protein assays
|
Module 2 Final Report Due
|
3
|
6
|
Tue May 3
|
ANS
|
HHH]
|
Transcript-level analysis
|
|
3
|
7
|
Thur May 5
|
ANS
|
HHH
|
Protein-level and wrap-up analysis
|
Module 3 Summary Due
|
3
|
8
|
Tue May 10
|
ANS
|
HHH
|
Student presentations
|
|
|
|
Thur May 12
|
|
|
No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
|
|