| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
note
|
|
|
|
Tue Feb 1
|
ANS
|
|
Orientation
|
* Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
|
|
|
| 1
|
1
|
Thur Feb 3
|
JN
|
CT
|
Amplify aptamer-encoding DNA
|
* M1D1 Lecture
* M1D1 Pre-Lab
|
| 1
|
2
|
Tue Feb 8
|
JN
|
CT
|
Purify aptamer-encoding DNA
|
* M1D2 Lecture
* M1D2 Pre-Lab
|
| 1
|
3
|
Thur Feb 10
|
JN
|
CT
|
Prepare RNA by IVT
|
* M1D3 Lecture
* M1D3 Pre-Lab
|
| 1
|
4
|
Tue Feb 15
|
JN
|
CT
|
Purify RNA and run affinity column
|
* M1D4 Lecture
* M1D4 Pre-Lab
|
| 1
|
5
|
Thur Feb 17
|
JN
|
CT
|
RNA to DNA by RT-PCR
|
* M1D5 Lecture
* M1D5 Pre-Lab
|
|
|
|
President's Day Feb 21
|
No lab Feb 22 or Feb 23
|
| 1
|
6
|
Thur Feb 24
|
JN
|
CT
|
Post-selection IVT and journal club
|
* M1D6 Lecture
* M1D6 Pre-Lab
|
| 1
|
7
|
Tue Mar 1
|
JN
|
CT
|
Aptamer binding assay
|
* M1D7 Lecture
* M1D7 Pre-Lab
|
| 1
|
8
|
Thur Mar 3
|
JN
|
CT
|
Journal club
|
* M1D8 Lecture
|
|
|
| 2
|
1
|
Tue Mar 8
|
RW
|
AR
|
Explore model system
|
Module 1 Draft Due
* M2D1 Lecture
* M2D1 Pre-Lab
|
| 2
|
2
|
Thur Mar 10
|
RW
|
AR
|
Complete DNA design
|
* M2D2 Lecture
* M2D2 Pre-Lab
|
| 2
|
3
|
Tue Mar 15
|
RW
|
AR
|
Prepare DNA for cloning
|
* M2D3 Lecture
* M2D3 Pre-Lab
|
| 2
|
4
|
Thur Mar 17
|
RW
|
AR
|
Ligate DNA and transform bacteria
|
* M2D4 Lecture
Note: much of lecture was on chalkboard today
* M2D4 Pre-Lab
|
|
|
|
Spring Break Mar 21-25
|
No lab all week
|
| 2
|
5
|
Tue Mar 29
|
RW
|
AR
|
Prepare candidate clones in model cell strain
|
* M2D5 Lecture
* M2D5 Pre-Lab
|
| 2
|
6
|
Thur Mar 31
|
RW
|
AR
|
Evaluate DNA and choose clone
|
Module 1 Final Report Due
* M2D6 Lecture
* M2D6 Pre-Lab
|
| 2
|
7
|
Tue Apr 5
|
RW
|
AR
|
Assay modified model system
|
* M2D7 Lecture
* M2D7 Pre-Lab
|
| 2
|
8
|
Thur Apr 7
|
RW
|
AR
|
Wrap-up analysis and modeling
|
* M2D8 Lecture
|
|
|
| 3
|
1
|
Tues Apr 12
|
ANS
|
HHH
|
Start-up biomaterials engineering
|
Module 2 Draft Due
* M3D1 Lecture
* M3D1 Pre-Lab
|
| 3
|
2
|
Thur Apr 14
|
ANS
|
HHH
|
Initiate cell culture
|
* M3D2 Lecture
|
|
|
|
Patriot's Day April 18-19
|
No lab Apr 19 or Apr 20
|
| 3
|
3
|
Thur Apr 21
|
ANS
|
HHH
|
Testing cell viability
|
* M3D3 Lecture
|
| 3
|
4
|
Tue Apr 26
|
ANS
|
HHH
|
Preparing cells for analysis
|
* M3D4 Lecture
* M3D4 Pre-Lab
|
| 3
|
5
|
Thur Apr 28
|
ANS
|
HHH
|
Initiating transcript and protein assays
|
Module 2 Final Report Due
* M3D5 Lecture
* M3D5 Pre-Lab
|
| 3
|
6
|
Tue May 3
|
ANS
|
HHH]
|
Transcript-level analysis
|
M3D6 Lecture
* M3D6 Pre-Lab
|
| 3
|
7
|
Thur May 5
|
ANS
|
HHH
|
Protein-level and wrap-up analysis
|
Module 3 Summary Due
|
| 3
|
8
|
Tue May 10
|
ANS
|
HHH
|
Student presentations
|
|
|
|
|
Thur May 12
|
|
|
No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
|
|
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