20.109(S11): Spring 2011 schedule
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| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 1 | ANS | Orientation | ||||
| 1 | 1 | Thur Feb 3 | JN | Amplify aptamer-encoding DNA | ||
| 1 | 2 | Tue Feb 8 | JN | Purify aptamer-encoding DNA | ||
| 1 | 3 | Thur Feb 10 | JN | Prepare RNA by IVT | ||
| 1 | 4 | Tue Feb 15 | JN | Purify RNA and run affinity column | ||
| 1 | 5 | Thur Feb 17 | JN | RNA to DNA by RT-PCR | ||
| President's Day Feb 21 | No lab Feb 22 or Feb 23 | |||||
|---|---|---|---|---|---|---|
| 1 | 6 | Thur Feb 24 | JN | Post-selection IVT and journal club | ||
| 1 | 7 | Tue Mar 1 | JN | Aptamer binding assay | ||
| 1 | 8 | Thur Mar 3 | JN | Journal club | ||
| 2 | 1 | Tue Mar 8 | RW | Explore model system | Module 1 Draft Due | |
| 2 | 2 | Thur Mar 10 | RW | Complete DNA design | ||
| 2 | 3 | Tue Mar 15 | RW | Prepare DNA for cloning | ||
| 2 | 4 | Thur Mar 17 | RW | Ligate DNA and transform bacteria | ||
| Spring Break Mar 21-25 | No lab all week | |||||
| 2 | 5 | Tue Mar 29 | RW | Prepare candidate clones in model cell strain | ||
| 2 | 6 | Thur Mar 31 | RW | Evaluate DNA and choose clone | Module 1 Final Report Due | |
| 2 | 7 | Tue Apr 5 | RW | Assay modified model system | ||
| 2 | 8 | Thur Apr 7 | RW | Wrap-up analysis and modeling | ||
| 3 | 1 | Tues Apr 12 | ANS | Start-up biomaterials engineering | Module 2 Draft Due | |
| 3 | 2 | Thur Apr 14 | ANS | Initiate cell culture | ||
| Patriot's Day April 18-19 | No lab Apr 19 or Apr 20 | |||||
| 3 | 3 | Thur Apr 21 | ANS | Testing cell viability | ||
| 3 | 4 | Tue Apr 26 | ANS | Preparing cells for analysis | ||
| 3 | 5 | Thur Apr 28 | ANS | Initiating transcript and protein assays | Module 2 Final Report Due | |
| 3 | 6 | Tue May 3 | ANS | Transcript-level analysis | ||
| 3 | 7 | Thur May 5 | ANS | Protein-level and wrap-up analysis | ||
| 3 | 8 | Tue May 10 | ANS | Student presentations | ||
| Thur May 12 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
