20.109(S11): Spring 2011 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 1	ANS		Orientation
1	1	Thur Feb 3	JN		Amplify aptamer-encoding DNA
1	2	Tue Feb 8	JN		Purify aptamer-encoding DNA
1	3	Thur Feb 10	JN		Prepare RNA by IVT
1	4	Tue Feb 15	JN		Purify RNA and run affinity column
1	5	Thur Feb 17	JN		RNA to DNA by RT-PCR
		President's Day Feb 21				No lab Feb 22 or Feb 23
1	6	Thur Feb 24	JN		Post-selection IVT and journal club
1	7	Tue Mar 1	JN		Aptamer binding assay
1	8	Thur Mar 3	JN		Journal club
2	1	Tue Mar 8	RW		Explore model system	Module 1 Draft Due
2	2	Thur Mar 10	RW		Complete DNA design
2	3	Tue Mar 15	RW		Prepare DNA for cloning
2	4	Thur Mar 17	RW		Ligate DNA and transform bacteria
		Spring Break Mar 21-25				No lab all week
2	5	Tue Mar 29	RW		Prepare candidate clones in model cell strain
2	6	Thur Mar 31	RW		Evaluate DNA and choose clone	font color = CC0000>Module 1 Final Report Due
2	7	Tue Apr 5	RW		Assay modified model system
2	8	Thur Apr 7	RW		Wrap-up analysis and modeling
3	1	Tues Apr 12	ANS		Start-up biomaterials engineering	Module 2 Draft Due
3	2	Thur Apr 14	ANS		Initiate cell culture
		Patriot's Day April 18-19				No lab Apr 19 or Apr 20
3	3	Thur Apr 21	ANS		Testing cell viability
3	4	Tue Apr 26	ANS		Preparing cells for analysis
3	5	Thur Apr 28	ANS		Initiating transcript and protein assays	Module 2 Final Report Due
3	6	Tue May 3	ANS		Transcript-level analysis
3	7	Thur May 5	ANS		Protein-level and wrap-up analysis
3	8	Tue May 10	ANS		Student presentations
		Thur May 12			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD