20.109(S12): Spring 2012 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	NOTES
		Tue Feb 7	ANS	JRR	Orientation	* Introductory Lecture (PDF) * Intro Pre-Lab, T/R
1	1	Thur Feb 9	JN	JRR	Amplify aptamer-encoding DNA	* M1D1 Lecture * M1D1 Pre-Lab * Writing tutor talk
1	2	Tue Feb 14	JN	JRR	Purify aptamer-encoding DNA	* M1D2 Lecture * M1D2 Pre-Lab * T/R WAC talk * W/F WAC talk
1	3	Thur Feb 16	JN	JRR	Prepare RNA by IVT	* M1D3 Lecture * M1D3 Pre-Lab * Oral presentation talk
		President's Day Feb 20				No lab Feb 21 or Feb 22
1	4	Thur Feb 23	JN	JRR	Purify RNA and run affinity column	* M1D4 Lecture * M1D4 Pre-Lab
1	5	Tue Feb 28	JN	JRR	RNA to DNA by RT-PCR	* M1D5 Lecture * M1D5 Pre-Lab
1	6	Thur Mar 1	JN	JRR	Post-selection IVT and journal club	* M1D6 Lecture * M1D6 Pre-Lab
1	7	Tue Mar 6	JN	JRR	Aptamer binding assay	* M1D7 Lecture * M1D7 Pre-Lab
1	8	Thur Mar 8	JN	JRR	Journal club	* M1D8 Lecture
3	1	Tue Mar 13	ANS	HHH	Start-up biomaterials engineering	* M3D1 Lecture * M3D1 Pre-Lab Module 1 Draft Due
2	1	Thur Mar 15	AJ	FF	Start-up protein engineering	* M2D1 Lecture * M2D1 Pre-Lab Module 1 Computational Assignment Due
2	2	Tue Mar 20	AJ	FF	Site-directed mutagenesis	* M2D2 Lecture * M2D2 Pre-Lab
2	3	Thur Mar 22	AJ	FF	Bacterial amplification of DNA	* M2D3 Lecture * M2D3 Pre-Lab
		Spring Break Mar 26-30				No lab all week
2	4	Tue Apr 3	AJ	FF	Prepare expression system	* M2D4 Lecture * M2D4 Pre-Lab
2	5	Thur Apr 5	AJ	FF	Induce protein and evaluate DNA	* M2D5 Lecture * M2D5 Pre-Lab Module 1 Final Report Due
2	6	Tue Apr 10	AJ	FF	Characterize protein expression	* M2D6 Lecture (got through slide 15) * M2D6 Pre-Lab
2	7	Thur Apr 12	AJ	FF	Assess protein function	* M2D7 Lecture * M2D7 Pre-Lab
		Patriot's Day April 16-17				No lab Apr 17 or Apr 18
3	2	Thur Apr 19	ANS	HHH	Initiate cell culture	* M3D2 Lecture
		Tue Apr 24	ANS	HHH		No lab or lecture. Module 2 Draft due MONDAY April 23rd by 1 pm. (Changed to April 24th.)
3	3	Thur Apr 26	ANS	HHH	Testing cell viability	* M3D3 Lecture
3	4	Tue May 1	ANS	HHH	Preparing cells for analysis	* M3D4 Lecture * M3D4 Pre-Lab
3	5	Thur May 3	ANS	HHH	Initiating transcript and protein assays	* M3D5 Lecture * M3D5 Pre-Lab
3	6	Tue May 8	ANS	HHH	Transcript-level analysis	Module 2 Final Report Due * M3D6 Lecture * M3D6 Pre-Lab
3	7	Thur May 10	ANS	HHH	Protein-level and wrap-up analysis	Module 3 Summary Due
3	8	Tue May 15	ANS	HHH	Student presentations
		Thur May 17			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD