20.109(S12): TA notes for orientation

Station 1

Up front at teaching bench:

• Two sets each
  • Cuvette box
  • 50 mL conical with water
  • 15 mL conical with XC
  • Pipet temporary waste container
• Agi extra pipets, tips

Station 2

TA runs this station. Cover:

• Utility of a dissecting microscope
• Turning on, adjusting light
• Reading magnification (dot at sides!)

• Each student should practice focusing!
  • Low, then high mag
  • Mess up focus before they start
  • Use hemacytometer as sample

• Taking a picture: zoom, save, playback

• Utility of a fluorescence microscope (if time)

Station 3

At the balance:

• Sorbitol in 50 mL conical tubes, ~ 2/3 full, X of them [tk check current]
• Check weigh boat and stir bar stocks, wash spatulas

All across from Yellow group bench:

• Signs posted: station 3 materials here, station 5 materials behind you: tk LINK PPT FILE
• 3 sets each
  • small beaker (150-250 mL)
  • small graduated cylinders (100 mL)
  • 0.5 L media bottle filled with distilled water, 2 sets only
  • 25 mL pipets, 1 per team plus 2 extra
  • pipet bulbs, 2 only

Station 4

Calibrate pH meter that day, using freshly poured calibration solutions the first of the two days. Hit "calibrate," go into the first solution until the reading stabilizes, hit "calibrate" again, and after all three solutions have been measured in this way, hit "X"tk CHECK

Have one test sorbitol solution available inside a 50 mL conical tube (prepared according to station 3).

Station 5

Specs should be turned on at the beginning of lab. Make sure the DU 730 wavelength scan is set to read between 300 and 900 nm.