20.109(S12): TA notes for orientation

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Station 1

Up front at teaching bench:

  • Two sets each
    • Cuvette box
    • 50 mL conical with water
    • 15 mL conical with XC (check w/Agi re: OD prep)
    • Pipet temporary waste container
  • Agi extra pipets, tips

Station 2

TA runs this station. Cover:

  • Utility of a dissecting microscope
  • Turning on, adjusting light
  • Reading magnification (dot at sides!)
  • Each student should practice focusing!
    • Low, then high mag
    • Mess up focus before they start
    • Use hemacytometer as sample
  • Taking a picture: zoom, save, playback
  • Utility of a fluorescence microscope (if time)

Station 3

At the balance:

  • Sorbitol in 50 mL conical tubes, ~ 2/3 full, 9 of them
  • Check weigh boat and stir bar stocks, wash spatulas

All across from Yellow group bench:

  • Signs posted: station 3 materials here, station 5 materials behind you: tk LINK PPT FILE
  • 3 sets each
    • small beaker (150-250 mL)
    • small graduated cylinders (100 mL)
    • 0.5 L media bottle filled with distilled water, 2 sets only
    • 25 mL pipets, 1 per team plus 2 extra
    • pipet bulbs, 2 only

Station 4

Calibrate pH meter that day, using freshly poured calibration solutions the first of the two days. Hit "calibrate," go into the first solution until the reading stabilizes, hit "calibrate" again, and after all three solutions have been measured in this way, hit "X"tk CHECK

Have one test sorbitol solution available inside a 50 mL conical tube (prepared according to station 3).

Station 5

Specs should be turned on at the beginning of lab. Make sure the DU 730 wavelength scan is set to read between 300 and 900 nm. Prepare one set of reference solutions (prepared according to station 1), including a blank, and leave them on top of the DU 640.

Station 6

Wipe down the equipment and the sample card with a damp paper towel to rid dust.

Station 7

Post cards with famous scientist names on the equipment indicated.

Station 8

Be prepared to give hints about where to find the safety shower, fire blanket, and spill kits.

Station 9

Be prepared to answer questions about this version of Excel. (Mostly instructor.)

Station 10

Be prepared to answer questions about this version of basic lab math. (Mostly instructor.)

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