20.109(S13):Context-setting and primer design (Day1)

Introduction

overall motivation

background about small subunit rRNA

bacteria-specific background

microsporidia-specific background

primer design basic background here or directly in protocols?

Protocols

Part 1: Lab practical

You and your partner may work together on the lab practical. (Note: this will not be the case for future quizzes.) You are of course welcome to give different answers should you disagree.

Part 2: Explore existing microsporidia-specific primers

Part 3: Design novel microsporidia-specific primers

For next time

Reagent list