20.109(S13):DNA cloning (Day4): Difference between revisions
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==Protocols== | ==Protocols== | ||
===Part 1: Purify 16S PCR band from gel=== | |||
===Part 2: Ligate purified product to vector arms=== | |||
===Part 3: Transform ligated product into cloning strain=== | |||
==For next time== | ==For next time== | ||
==Reagent list== | ==Reagent list== |
Revision as of 11:20, 6 December 2012
Introduction
background on this cloning system -- vector arms and strain
transformation