20.109(S13): Spring 2013 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	NOTES
		Tue/Wed Feb 5/6	ANS		Orientation	* Introductory Lecture (PDF) * Intro Pre-Lab, T/R
1	1	Thur/Fri Feb 7/8	JR		Context-setting and primer design
1	2	Tue/Wed Feb 12/13	JR		DNA extraction
1	3	Thur/Fri Feb 14/15	JR		PCR and paper discussion
		President's Day Feb 18				No lab Feb 19 or Feb 20
1	4	Thur/Fri Feb 21/22	JR		DNA cloning
1	5	Tue/Wed Feb 26/27	JR		DNA sequencing and primer analysis
1	6	Thur/Fri Feb 28/ Mar 1	JR		Journal club I
1	7	Tue/Wed Mar 5/6	JR		Phylogenetic analysis
1	8	Thur/Fri Mar 7/8	NO LECTURE TODAY		Journal club II
2	1	Tue/Wed Mar 12/13	AJ		Design mutant
2	2	Thur/Fri Mar 14/15	AJ		Site-directed mutagenesis
2	3	Tue/Wed Mar 19/20	AJ		Bacterial amplification of DNA
2	4	Thur/Fri Mar 21/22	AJ		Prepare expression system
		Spring Break Mar 25-29				No lab all week
2	5	Tue/Wed Apr 2/3	AJ		Induce protein and evaluate DNA
2	6	Thur/Fri Apr 4/5	AJ		Purify protein
2	7	Tue/Wed Apr 9/10	AJ		Characterize protein expression
2	8	Thur/Fri Apr 11/12	AJ		Assess protein function
		Patriot's Day April 15-16				No lab Apr 16 or Apr 17
3	1	Thur/Fri Apr 18/19	ANS		Design experiment
3	2	Tue/Wed Apr 23/24	ANS		Initiate cell culture
3	3	Thur/Fri Apr 25/26	ANS		Testing cell viability
3	4	Tue/Wed Apr 30/May 1	ANS		Preparing cells for analysis
3	5	Thur/Fri May 2/3	ANS		Initiating transcript and protein assays
3	6	Tue/Wed May 7/8	ANS		Transcript-level analysis
3	7	Thur/Fri May 9/10	ANS		Protein-level and wrap-up analysis
3	8	Tue/Wed May 14/15	ANS		Student presentations
		Thur May 16			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD