20.109(S13): Spring 2013 schedule
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| MODULE | DAY | DATE | LECTURER | TA | LAB | NOTES |
| Tue/Wed Feb 5/6 | ANS | Orientation | * Introductory Lecture (PDF) * Intro Pre-Lab, T/R | |||
| 1 | 1 | Thur/Fri Feb 7/8 | JR | Context-setting and primer design | ||
| 1 | 2 | Tue/Wed Feb 12/13 | JR | DNA extraction | ||
| 1 | 3 | Thur/Fri Feb 14/15 | JR | PCR and paper discussion | ||
| President's Day Feb 18 | No lab Feb 19 or Feb 20 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur/Fri Feb 21/22 | JR | DNA cloning | ||
| 1 | 5 | Tue/Wed Feb 26/27 | JR | DNA sequencing and primer analysis | ||
| 1 | 6 | Thur/Fri Feb 28/ Mar 1 | JR | Journal club I | ||
| 1 | 7 | Tue/Wed Mar 5/6 | JR | Phylogenetic analysis | ||
| 1 | 8 | Thur/Fri Mar 7/8 | NO LECTURE TODAY | Journal club II | ||
| 2 | 1 | Tue/Wed Mar 12/13 | AJ | MM | Design mutant | Module 1 Draft Due |
| 2 | 2 | Thur/Fri Mar 14/15 | AJ | MM | Site-directed mutagenesis | |
| 2 | 3 | Tue/Wed Mar 19/20 | AJ | MM | Bacterial amplification of DNA | |
| 2 | 4 | Thur/Fri Mar 21/22 | AJ | MM | Prepare expression system | |
| Spring Break Mar 25-29 | No lab all week | |||||
| 2 | 5 | Tue/Wed Apr 2/3 | AJ | MM | Induce protein and evaluate DNA | |
| 2 | 6 | Thur/Fri Apr 4/5 | AJ | Purify protein | Module 1 Revision Due | |
| 2 | 7 | Tue/Wed Apr 9/10 | AJ | MM | Characterize protein expression | |
| 2 | 8 | Thur/Fri Apr 11/12 | AJ | MM | Assess protein function | |
| Patriot's Day April 15-16 | No lab Apr 16 or Apr 17 | |||||
| 3 | 1 | Thur/Fri Apr 18/19 | ANS | Design experiment | ||
| 3 | 2 | Tue/Wed Apr 23/24 | ANS | Initiate cell culture | Module 2 Report Due MONDAY APRIL 22 by 5 pm for all. | |
| 3 | 3 | Thur/Fri Apr 25/26 | ANS | Testing cell viability | ||
| 3 | 4 | Tue/Wed Apr 30/May 1 | ANS | Preparing cells for analysis | ||
| 3 | 5 | Thur/Fri May 2/3 | ANS | Initiating transcript and protein assays | ||
| 3 | 6 | Tue/Wed May 7/8 | ANS | Transcript-level analysis | ||
| 3 | 7 | Thur/Fri May 9/10 | ANS | Protein-level and wrap-up analysis | Module 3 Summary Due | |
| 3 | 8 | Tue/Wed May 14/15 | ANS | Student presentations | ||
| Thur May 16 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
