20.109(S13): TA notes for module 1
From OpenWetWare
Coming soon!
General notes
See also GoogleDoc for aliquoting amounts.
Scheme:
Key preparation:
Day-by-day
Day 1
Materials required:
Day of Lab (R/F):
After Lab:
How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.
Day 2
Materials required:
- Bird samples! At 100 μL each in a 2 mL tube
- Aliquots of components from QiaAMP stool kit (see GoogleDoc)
- Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
- InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one
Day of Lab (R/F):
- Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
- Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
- Either set up second heat block at 56 °C or turn on the small oven (may be easiest)
After Lab:
How it went:
