20.109(S14):Begin Western protein analysis (Day2): Difference between revisions
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==Introduction== | ==Introduction== | ||
Topic 1: Reiterate purpose of WB, now some details about cell lines | |||
Topic 2: Basis of WB, including background on antibodies | |||
==Protocols== | ==Protocols== | ||
Revision as of 19:31, 5 March 2014
Introduction
Topic 1: Reiterate purpose of WB, now some details about cell lines
Topic 2: Basis of WB, including background on antibodies
Protocols
Part 1: Prepare cell lysates
Part 2: Measure protein content
Part 3: Separate proteins by SDS-PAGE
Part 4: Transfer proteins to membrane
For next time
Reagent list
write something here or not accessible to edit
Next Day: Choose system conditions and paper discussion Previous Day: Introduction to cell strains and plating
