20.109(S14):Begin Western protein analysis (Day2)

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20.109(S14): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2014        Assignments       
Module 1        Module 2        Module 3              

Introduction

Protocols

Part 1: Prepare cell lysates

Part 2: Measure protein content

Part 3: Separate proteins by SDS-PAGE

Part 4: Transfer proteins to membrane

For next time

Reagent list

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Next Day: Choose system conditions and paper discussion Previous Day: Introduction to cell strains and plating