20.109(S14): Choose system conditions and paper discussion (Day3): Difference between revisions
| Line 29: | Line 29: | ||
*Let's pay special attention to the Methods section, since you will be writing one during Module 2! | *Let's pay special attention to the Methods section, since you will be writing one during Module 2! | ||
**What best practices for Methods section writing do the authors follow? For example, do they write clear topic sentences of appropriate scope? | **What best practices for Methods section writing do the authors follow? For example, do they write clear topic sentences of appropriate scope? | ||
**What is the | **What is the authors' strategy for sub-section groupings? What is the role of the first sub-section? | ||
**What methods do the authors seem to assume that most readers will be familiar with? | **What methods do the authors seem to assume that most readers will be familiar with? | ||
**What methods do the authors seem to assume need more detailed definition or citation? | **What methods do the authors seem to assume need more detailed definition or citation? | ||
Revision as of 09:46, 15 March 2014
Introduction
brief context
topic 1: restriction enzymes
topic 2: design considerations in a repair report assay
Protocols
Part 1: Paper discussion
Coming soon!
Technical Background
The main technical topic that may be unfamiliar to (some of) you is the use of short interfering RNA (siRNA). RNA interference, or RNAi, is a post-transcriptional silencing mechanism. You may have heard of gene "knock-outs" before, and in fact xrs6 are essentially Ku80 knock-outs: the DNA sequence is deleted or modified so as to be non-functional. In contrast, RNAi is usually not as potent, and hence is called gene "knock-down." Here the DNA sequence is normal, and mRNA is transcribed – but it is bound up or destroyed before it can be translated. You can read more about the intricacies of RNAi at Scitable.
Discussion Topics
Writing
- How does this abstract style differ from the one you have previously encountered – and also explicitly been taught – in 20.109? How is it the same? What are the pros and cons of each format? As a whole, did the abstract make you want to read the paper?
- This Introduction is short but packs a punch. With the 20.109 guidelines in mind, locate the key elements of an introductory section here. What one sentence best defines the research gap?
- Let's pay special attention to the Methods section, since you will be writing one during Module 2!
- What best practices for Methods section writing do the authors follow? For example, do they write clear topic sentences of appropriate scope?
- What is the authors' strategy for sub-section groupings? What is the role of the first sub-section?
- What methods do the authors seem to assume that most readers will be familiar with?
- What methods do the authors seem to assume need more detailed definition or citation?
- Are there any changes you would suggest the authors make?
Content
Part 2: Reverse engineering pMax-BFP-MCS
Coming later!
Part 3: Choose system conditions
On today's Talk page...
For next time
digest planning and calculations in advance
Reagent list
Just your brains!
Next Day: Complete Western and prepare damaged DNA Previous Day: Begin Western protein analysis
