20.109(S14): Spring 2014 schedule: Difference between revisions

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| [[User:Christopher Bandoro| Chris]]
| [[User:Christopher Bandoro| Chris]]
| [[20.109(S14):DNA sequencing (Day5)| DNA sequencing]]
| [[20.109(S14):DNA sequencing (Day5)| DNA sequencing]]
|<font color=green>lab quiz</font color><br>[[Media:S14_M1D5_AKR_TR_filled.pdf | T/R Pre-lab]]<br>[[Media:S14_M1D5_AKR_WF_filled.pdf | W/F Pre-lab]]
|<font color=green>lab quiz</font color><br>[[Media:S14_M1D5-Lecture.pptx.pdf | Lecture]]<br>[[Media:Module1_Lecture5_LectureOutline.docx | L5 notes]]<br>[[Media:S14_M1D5_AKR_TR_filled.pdf | T/R Pre-lab]]<br>[[Media:S14_M1D5_AKR_WF_filled.pdf | W/F Pre-lab]]
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| [[User:Christopher Bandoro| Chris]]
| [[User:Christopher Bandoro| Chris]]
| [[20.109(S14):Journal club I (Day6)| Journal club I]]
| [[20.109(S14):Journal club I (Day6)| Journal club I]]
|
| [[Media:S14_M1D6-Lecture.pptx.pdf | Lecture]]<br>[[Media:Module1_Lecture6_LectureOutline.doc | L6 notes]]
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| [[User:Christopher Bandoro| Chris]]
| [[User:Christopher Bandoro| Chris]]
| [[20.109(S14):Phylogenetic and primer analyses (Day7)| Phylogenetic and primer analyses]]
| [[20.109(S14):Phylogenetic and primer analyses (Day7)| Phylogenetic and primer analyses]]
|
| [[Media: S14_M1D7-Lecture.pptx.pdf | Lecture]]<br>[[Media:M1D7_S14_SKH_final.pdf|T/R Pre-lab]]<br>[[Media:S14_M1D7-ANS.pdf | W/F Pre-Lab]]
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| [[User:Christopher Bandoro| Chris]]
| [[User:Christopher Bandoro| Chris]]
| [[20.109(S14):Journal club II (Day8)| Journal club II]]
| [[20.109(S14):Journal club II (Day8)| Journal club II]]
|<font color=green>lab quiz</font color>
|<font color=green>lab quiz</font color><br>[[Media: S14_M1D8-Lecture-AdditionalSlides.pptx.pdf | Lecture]]<br>[[Media: S14M1-JonR_Lecture7-8_Outline.doc | L7/8 notes]]<br>[[Media:20.109_Abstract_SP14.pdf | WAC lecture, abstracts]]
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| colspan="7" |  
| colspan="7" |  
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| [http://web.mit.edu/be/people/samson.shtml LS]
| [http://web.mit.edu/be/people/samson.shtml LS]
| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):Introduction to system components (Day1)| Introduction to system components]]
| [[20.109(S14):Introduction to cell strains and plating (Day1)| Introduction to cell strains and plating]]
| <br><font color = CC0000><b>Draft Mod 1 Abstract and Summary Due</b></font color><br>
| [[Media:20109_S14-M2Lec1_LSamson.pdf | Lecture]]<br>[[Media:M2D1_S14_SKH_final.pdf|T/R Prelab]]<br>[[Media:S14_M2D1-ANS.pdf | W/F Pre-Lab]]<br>[[Media:20.109_IMRD_SP14a.pdf | WAC lecture, IMRD]]<br><font color = CC0000><b>Draft Mod 1 Abstract and Summary Due WED 1 PM</b></font color><br>
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| [http://web.mit.edu/be/people/samson.shtml LS]
| [http://web.mit.edu/be/people/samson.shtml LS]
| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):Cell plating and plasmid digestion (Day2)| Cell plating and plasmid digestion]]
| [[20.109(S14):Begin Western protein analysis (Day2)| Begin Western protein analysis]]
|
|[[Media:20109_S14-M2Lec2_LSamson.pdf | Lecture]]<br>[[Media:M2D2_S14_SKH_final.pdf|T/R Prelab]]<br>[[Media:S14_M2D2-ANS.pdf | W/F Pre-Lab]]
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| [http://web.mit.edu/be/people/samson.shtml LS]
| [http://web.mit.edu/be/people/samson.shtml LS]
| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14): Begin Western protein analysis (Day3)| Begin Western protein analysis]]
| [[20.109(S14): Choose system conditions and paper discussion (Day3)| Choose system conditions and paper discussion]]
|<br><font color = CC0000><b>Mod 1 Memo Due</b></font color><br><font color=green>lab quiz</font color>
|<br><font color = CC0000><b>Mod 1 Memo Due</b></font color><br><font color=green>lab quiz</font color><br>[[Media:20109_S14-M2Lec3_LSamson.pdf | Lecture]]<br>[[Media:M2D3_S14_SKH_final.pdf | T/R Pre-Lab]]<br>[[Media:S14_M2D3-ANS.pdf | W/F Pre-Lab]]
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| [http://web.mit.edu/be/people/samson.shtml LS]
| [http://web.mit.edu/be/people/samson.shtml LS]
| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):Complete Western and plasmid purification (Day4)| Complete Western and plasmid purification]]
| [[20.109(S14):Complete Western and prepare damaged DNA (Day4)| Complete Western and prepare damaged DNA]]
|
| [[Media:20109_S14-M2Lec4_LSamson.pdf | Lecture]]<br>[[Media:M2D4_S14_SKH_final.pdf| T/R Pre-lab]]<br>[[Media:S14_M2D4-ANS.pdf | W/F Pre-Lab]]
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| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):Cell preparation for DNA repair assays (Day5)| Cell preparation for DNA repair assays]]
| [[20.109(S14):Cell preparation for DNA repair assays (Day5)| Cell preparation for DNA repair assays]]
|<font color=green>lab quiz</font color>
|<font color=green>lab quiz</font color><br>[[Media:20109_S14-M2Lec5_LSamson.pdf | Lecture]]<br>[[Media:M2D5_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M2D5-ANS.pdf | W/F Pre-Lab]]
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| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):DNA repair assays(Day6)| DNA repair assays]]
| [[20.109(S14):DNA repair assays(Day6)| DNA repair assays]]
|<br><font color = CC0000><b>Final Mod 1 Abstract and Summary Due</b></font color><br>
|[[Media:20109_S14-M2Lec6_LSamson.pdf | Lecture]]<br>[[Media:M2D6_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M2D6-ANS.pdf | W/F Pre-Lab]]<br><font color = CC0000><b>Final Mod 1 Abstract and Summary Due</b></font color><br>
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| [[User: Suhani Vora|SV]]
| [[User: Suhani Vora|SV]]
| [[20.109(S14):Data analysis (Day7)| Data analysis]]
| [[20.109(S14):Data analysis (Day7)| Data analysis]]
|<font color=green>lab quiz</font color>
|<font color=green>lab quiz</font color><br>[[Media:ZDN_Module_Lecture_4-8-14.pdf| Lecture]]<br>[[Media:M2D7_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M2D7-ANS.pdf | W/F Pre-Lab]]
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Design experiment (Day1)| Design experiment]]
| [[20.109(S14):Design experiment (Day1)| Design experiment]]
|  
| [[Media: 20109_S14-M3Lec1_AStachowiak.pdf | Lecture]]<br>[[Media: M3D1_S14_SKH_final.pdf | T/R Pre-Lab]] <br>[[Media:S14_M3D1-ANS.pdf | W/F Pre-Lab]]
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Initiate cell culture (Day2)| Initiate cell culture]]
| [[20.109(S14):Initiate cell culture (Day2)| Initiate cell culture]]
| <font color=green>lab quiz</font color>
| <font color=green>lab quiz</font color><br>[[Media: 20109_S14-M3Lec2_AStachowiak.pdf | Lecture]]<br>
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Testing cell viability (Day3)| Testing cell viability]]
| [[20.109(S14):Testing cell viability (Day3)| Testing cell viability]]
|  
| [[Media: 20109_S14-M3Lec3_AStachowiak.pdf | Lecture]]<br>
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Preparing cells for analysis (Day4)| Preparing cells for analysis]]
| [[20.109(S14):Preparing cells for analysis (Day4)| Preparing cells for analysis]]
| <font color=green>lab quiz</font color>
| <font color=green>lab quiz</font color><br>[[Media: 20109_S14-M3Lec4_AStachowiak.pdf | Lecture]]<br>[[Media:M3D4_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M3D4-ANS.pdf | W/F Pre-Lab]]
|--
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|  
|  
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]]
| [[20.109(S14):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]]
|  
| [[Media: 20109_S14-M3Lec5_AStachowiak.pdf | Lecture]]<br>[[Media:M3D5_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M3D5-ANS.pdf | W/F Pre-Lab]]
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Transcript-level analysis (Day6)| Transcript-level analysis]]
| [[20.109(S14):Transcript-level analysis (Day6)| Transcript-level analysis]]
| <font color=green>lab quiz</font color>
| <font color=green>lab quiz</font color><br>[[Media: 20109_S14-M3Lec6_AStachowiak.pdf | Lecture]]<br>[[Media:S14_M3D6-ANS-TR.pdf|T/R Pre-Lab]]<br>[[Media:S14_M3D6-ANS.pdf | W/F Pre-Lab]]
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| [[User:Reginald K. Avery|RKA]]
| [[User:Reginald K. Avery|RKA]]
| [[20.109(S14):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
| [[20.109(S14):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
| <br><font color = CC0000><b>Mod 3 Mini-report Due</b></font color><br>
| <br><font color = CC0000><b>Mod 3 Mini-report Due</b></font color><br>[[Media:M3D7_S14_SKH_final.pdf|T/R Pre-Lab]]<br>[[Media:S14_M3D7-ANS.pdf | W/F Pre-Lab]]
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| No lab session. Meet in '''TBD''' from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.
| No lab session. Meet in '''16-220''' from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.
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|}
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Latest revision as of 10:41, 9 May 2014


20.109(S14): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2014        Assignments       
Module 1        Module 2        Module 3              

MODULE DAY DATE LECTURER TA LAB NOTES
Tue/Wed Feb 4/5 ANS Chris Orientation Introductory Lecture (PDF)
T/R Pre-Lab
W/F Pre-Lab
1 1 Thur/Fri Feb 6/7 JR Chris Microbial DNA extraction lab orientation quiz
Lecture
L1 Notes
T/R Pre-Lab
W/F Pre-Lab
1 2 Tue/Wed Feb 11/12 JR Chris Diagnostic primer design Lecture
L2 Notes
T/R Pre-lab
W/F Pre-Lab
1 3 Thur/Fri Feb 13/14 JR Chris 16S PCR and paper discussion lab quiz
Lecture
L3 Notes
T/R Pre-lab
W/F Pre-Lab
WAC lecture, talks
President's Day Feb 17 No lab Feb 18 or Feb 19
1 4 Thur/Fri Feb 20/21 JR Chris DNA cloning L4 Notes
T/R Pre-lab
W/F Pre-Lab
WAC lecture, figures
1 5 Tue/Wed Feb 25/26 JR Chris DNA sequencing lab quiz
Lecture
L5 notes
T/R Pre-lab
W/F Pre-lab
1 6 Thur/Fri Feb 27/28 JR Chris Journal club I Lecture
L6 notes
1 7 Tue/Wed Mar 4/5 JR Chris Phylogenetic and primer analyses Lecture
T/R Pre-lab
W/F Pre-Lab
1 8 Thur/Fri Mar 6/7 JR Chris Journal club II lab quiz
Lecture
L7/8 notes
WAC lecture, abstracts
2 1 Tue/Wed Mar 11/12 LS SV Introduction to cell strains and plating Lecture
T/R Prelab
W/F Pre-Lab
WAC lecture, IMRD
Draft Mod 1 Abstract and Summary Due WED 1 PM
2 2 Thur/Fri Mar 13/14 LS SV Begin Western protein analysis Lecture
T/R Prelab
W/F Pre-Lab
2 3 Tue/Wed Mar 18/19 LS SV Choose system conditions and paper discussion
Mod 1 Memo Due
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
2 4 Thur/Fri Mar 20/21 LS SV Complete Western and prepare damaged DNA Lecture
T/R Pre-lab
W/F Pre-Lab
Spring Break Mar 24-28 No lab all week
2 5 Tue/Wed Apr 1/2 LS SV Cell preparation for DNA repair assays lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
2 6 Thur/Fri Apr 3/4 LS SV DNA repair assays Lecture
T/R Pre-Lab
W/F Pre-Lab
Final Mod 1 Abstract and Summary Due
2 7 Tue/Wed Apr 8/9 LS SV Data analysis lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
3 1 Thur/Fri Apr 10/11 ANS RKA Design experiment Lecture
T/R Pre-Lab
W/F Pre-Lab
3 2 Tue/Wed Apr 15/16 ANS RKA Initiate cell culture lab quiz
Lecture
3 3 Thur/Fri Apr 17/18 ANS RKA Testing cell viability Lecture
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
Mod 2 Report Due
3 4 Thu/Fri Apr 24/25 ANS RKA Preparing cells for analysis lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
Tue/Wed Apr 29/30 No lecture or lab today.
3 5 Thur/Fri May 1/2 ANS RKA Initiating transcript and protein assays Lecture
T/R Pre-Lab
W/F Pre-Lab
3 6 Tue/Wed May 6/7 ANS RKA Transcript-level analysis lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
3 7 Thur/Fri May 8/9 ANS RKA Protein-level and wrap-up analysis
Mod 3 Mini-report Due
T/R Pre-Lab
W/F Pre-Lab
3 8 Tue/Wed May 13/14 ANS RKA Student presentations
Thur May 15 No lab session. Meet in 16-220 from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.


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