20.109(S14): TA notes for module 2: Difference between revisions
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'''Scheme:''' | '''Scheme:''' | ||
Each pair of students will test K1 and xrs6 cells for Ku80 production. They will then assess the ability of K1, xrs6, and DNA-PKcs inhibited cells to repair DNA damage via plasmid based assay and flow cytometry. | |||
'''Key preparation:''' | '''Key preparation:''' | ||
Revision as of 21:58, 11 March 2014
General notes
I need to figure out the quiz dates.
See also GoogleDoc for aliquoting amounts.
Scheme: Each pair of students will test K1 and xrs6 cells for Ku80 production. They will then assess the ability of K1, xrs6, and DNA-PKcs inhibited cells to repair DNA damage via plasmid based assay and flow cytometry.
Key preparation:
Cells:
- Prepare T25 flasks of K1 and xrs6 for every team
Westerns:
- Prepare transfer buffer
DNA plasmids:
- Aliquot plasmids
Lipofection: Flow cytometry:
Note to TA:
- While you read the wiki, test each link, and make any link descriptions bold that are not already.
- As you read the protocols, add reagent calculations to the GoogleDoc as needed.
Day-by-day
Day 1
Materials required: None
Day of Lab (T/W):
After Lab:
How it went:
Day 2
Materials required: Please read as much as you can of Parts 1-3 and create an automated worksheet (e.g., in Excel) that will perform the required calculations for the day. A partially prepared worksheet is linked here. We will NOT collect your final worksheet this time; it is only for your benefit.
Day of Lab (R/F):
How it went:
Day 3
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 4
Materials required:
Day of Lab (R/F):
After Lab:
How it went:
Day 5
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 6
Materials required:
Day of Lab (R/F):
After Lab:
How it went:
Day 7
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 8
Materials required:
Day of Lab (R/F):
After Lab:
How it went:
