20.109(F07):Module 1 - Revision history

http://www.openwetware.org/index.php?title=20.109(F07):Module_1&feed=atom&action=history 20.109(F07):Module 1 - Revision history 2013-05-21T19:16:15Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=144610&oldid=prev Nkuldell: /* Module 1 */ 2007-08-24T10:14:35Z

<p><span class="autocomment">Module 1</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:14, 24 August 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 10:</td> <td colspan="2" class="diff-lineno">Line 10:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''TA:''' [[Laure-Anne Ventouras]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''TA:''' [[Laure-Anne Ventouras]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In this experiment, we will consider the genome of a virus, namely the bacteriophage M13. M13 is a self-assembling nano-machine with a compact genome that has been optimized by evolution to commandeer its bacterial host. Approximately 1000 new viruses are generated from a single infection event.  Imagine harnessing this production. What could we build and what  natural processes could we better understand? One approach we’ll take is to modify the existing genome in a subtle but useful way, namely by adding a <del class="diffchange diffchange-inline">peptide</del>-tag that <del class="diffchange diffchange-inline">can be presented on </del>the bacteriophage coat. We’ll examine how this modification affects the coat protein’s expression and overall phage production. Another approach we’ll take is to start from scratch, undertaking a full throttle redesign of the bacteriophage genome. We’ll employ a commercial DNA synthesis company to compile the redesigned genomic program and then we’ll see if it encoded infective M13 and if the genome of the bacterial host affects bacteriophage production. Through these investigations we’ll ask: is nature’s M13 genome “perfect” or can we do better?   </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In this experiment, we will consider the genome of a virus, namely the bacteriophage M13. M13 is a self-assembling nano-machine with a compact genome that has been optimized by evolution to commandeer its bacterial host. Approximately 1000 new viruses are generated from a single infection event.  Imagine harnessing this production. What could we build and what  natural processes could we better understand? One approach we’ll take is to modify the existing genome in a subtle but useful way, namely by adding a <ins class="diffchange diffchange-inline">useful sequence</ins>-tag that <ins class="diffchange diffchange-inline">modifies </ins>the bacteriophage coat. We’ll examine how this modification affects the coat protein’s expression and overall phage production. Another approach we’ll take is to start from scratch, undertaking a full throttle redesign of the bacteriophage genome. We’ll employ a commercial DNA synthesis company to compile the redesigned genomic program and then we’ll see if it encoded infective M13 and if the genome of the bacterial host affects bacteriophage production. Through these investigations we’ll ask: is nature’s M13 genome “perfect” or can we do better?   </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Macintosh HD-Users-nkuldell-Desktop-GnmEng coverart S07.jpg|thumb|500px|center|M13-coated coli from M. Russel<br> Map of M13 genome from M. Blaber]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Macintosh HD-Users-nkuldell-Desktop-GnmEng coverart S07.jpg|thumb|500px|center|M13-coated coli from M. Russel<br> Map of M13 genome from M. Blaber]]</div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=137874&oldid=prev Nkuldell at 15:54, 5 August 2007 2007-08-05T15:54:03Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:54, 5 August 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 24:</td> <td colspan="2" class="diff-lineno">Line 24:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to the requirements for the genome engineering [[20.109(F07):_Genome_engineering_assessment| portfolio]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to the requirements for the genome engineering [[20.109(F07):_Genome_engineering_assessment| portfolio]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109(F07):Module 1:RefactorM13 | working page]] for M13 refactoring</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109(F07):Module 1:RefactorM13 | working page]] for M13 refactoring</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): TA's notes for module 1| TA notes, mod 1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): TA's notes for module 1| TA notes, mod 1]]</div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=130122&oldid=prev Nkuldell at 12:21, 15 July 2007 2007-07-15T12:21:10Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:21, 15 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''TA:'''  </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''TA:''' <ins class="diffchange diffchange-inline">[[Laure-Anne Ventouras]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this experiment, we will consider the genome of a virus, namely the bacteriophage M13. M13 is a self-assembling nano-machine with a compact genome that has been optimized by evolution to commandeer its bacterial host. Approximately 1000 new viruses are generated from a single infection event.  Imagine harnessing this production. What could we build and what  natural processes could we better understand? One approach we’ll take is to modify the existing genome in a subtle but useful way, namely by adding a peptide-tag that can be presented on the bacteriophage coat. We’ll examine how this modification affects the coat protein’s expression and overall phage production. Another approach we’ll take is to start from scratch, undertaking a full throttle redesign of the bacteriophage genome. We’ll employ a commercial DNA synthesis company to compile the redesigned genomic program and then we’ll see if it encoded infective M13 and if the genome of the bacterial host affects bacteriophage production. Through these investigations we’ll ask: is nature’s M13 genome “perfect” or can we do better?   </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this experiment, we will consider the genome of a virus, namely the bacteriophage M13. M13 is a self-assembling nano-machine with a compact genome that has been optimized by evolution to commandeer its bacterial host. Approximately 1000 new viruses are generated from a single infection event.  Imagine harnessing this production. What could we build and what  natural processes could we better understand? One approach we’ll take is to modify the existing genome in a subtle but useful way, namely by adding a peptide-tag that can be presented on the bacteriophage coat. We’ll examine how this modification affects the coat protein’s expression and overall phage production. Another approach we’ll take is to start from scratch, undertaking a full throttle redesign of the bacteriophage genome. We’ll employ a commercial DNA synthesis company to compile the redesigned genomic program and then we’ll see if it encoded infective M13 and if the genome of the bacterial host affects bacteriophage production. Through these investigations we’ll ask: is nature’s M13 genome “perfect” or can we do better?   </div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127584&oldid=prev Nkuldell at 02:34, 4 July 2007 2007-07-04T02:34:44Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 02:34, 4 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 22:</td> <td colspan="2" class="diff-lineno">Line 22:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Probe western| Module 1 Day 7: Probe western]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Probe western| Module 1 Day 7: Probe western]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Module 1 oral presentations| Module 1 Day 8: Oral Presentations]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Module 1 oral presentations| Module 1 Day 8: Oral Presentations]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>direct link to genome engineering [[20.109<del class="diffchange diffchange-inline">%28S07%29</del>:<del class="diffchange diffchange-inline">_Genome_engineering_essay</del>| <del class="diffchange diffchange-inline">essay</del>]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>direct link to <ins class="diffchange diffchange-inline">the requirements for the </ins>genome engineering [[20.109<ins class="diffchange diffchange-inline">(F07)</ins>:<ins class="diffchange diffchange-inline">_Genome_engineering_assessment</ins>| <ins class="diffchange diffchange-inline">portfolio</ins>]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109(F07):Module 1:RefactorM13 | working page]] for M13 refactoring</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109(F07):Module 1:RefactorM13 | working page]] for M13 refactoring</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127307&oldid=prev Nkuldell at 10:31, 3 July 2007 2007-07-03T10:31:38Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:31, 3 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 23:</td> <td colspan="2" class="diff-lineno">Line 23:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Module 1 oral presentations| Module 1 Day 8: Oral Presentations]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Module 1 oral presentations| Module 1 Day 8: Oral Presentations]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to genome engineering [[20.109%28S07%29:_Genome_engineering_essay| essay]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to genome engineering [[20.109%28S07%29:_Genome_engineering_essay| essay]]<br></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">direct link to [[20.109(F07):Module 1:RefactorM13 | working page]] for M13 refactoring</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): TA's notes for module 1| TA notes, mod 1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): TA's notes for module 1| TA notes, mod 1]]</div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127258&oldid=prev Nkuldell at 02:18, 3 July 2007 2007-07-03T02:18:23Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 02:18, 3 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(F07): Western analysis| Module 1 Day <del class="diffchange diffchange-inline">5</del>: Western analysis]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[20.109(F07): M13.1 | Module 1 Day 5: M13.1]]<br></ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(F07): Probe western| Module 1 Day <del class="diffchange diffchange-inline">6</del>: Probe western]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(F07): Western analysis| Module 1 Day <ins class="diffchange diffchange-inline">6</ins>: Western analysis]]<br></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(F07): Probe western| Module 1 Day <ins class="diffchange diffchange-inline">7</ins>: Probe western<ins class="diffchange diffchange-inline">]]<br></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[20.109(F07): Module 1 oral presentations| Module 1 Day 8: Oral Presentations</ins>]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to genome engineering [[20.109%28S07%29:_Genome_engineering_essay| essay]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to genome engineering [[20.109%28S07%29:_Genome_engineering_essay| essay]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127253&oldid=prev Nkuldell at 01:50, 3 July 2007 2007-07-03T01:50:39Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:50, 3 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): Probe western| Module 1 Day 6: Probe western]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): Probe western| Module 1 Day 6: Probe western]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>direct link to [[20.109%28S07%29:_Genome_engineering_essay<del class="diffchange diffchange-inline">]]<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>direct link to <ins class="diffchange diffchange-inline">genome engineering </ins>[[20.109%28S07%29:_Genome_engineering_essay| <ins class="diffchange diffchange-inline">essay</ins>]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">direct link to [[20.109:Module 1:RefactorM13</del>| <del class="diffchange diffchange-inline">M13 refactoring workpage</del>]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): TA's notes for module 1| TA notes, mod 1]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): TA's notes for module 1| TA notes, mod 1]]</div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127173&oldid=prev Nkuldell at 21:03, 2 July 2007 2007-07-02T21:03:36Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:03, 2 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 17:</td> <td colspan="2" class="diff-lineno">Line 17:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Probe western| Module 1 Day 6: Probe western]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Probe western| Module 1 Day 6: Probe western]]<br></div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127150&oldid=prev Nkuldell at 20:09, 2 July 2007 2007-07-02T20:09:08Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:09, 2 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 16:</td> <td colspan="2" class="diff-lineno">Line 16:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07):Start-up genome engineering | Module 1 Day 1: Start-up genome engineering]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07):Start-up genome engineering | Module 1 Day 1: Start-up genome engineering]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Western analysis| Module 1 Day 5: Western analysis]]<br></div></td></tr>  </table>

Nkuldell http://www.openwetware.org/index.php?title=20.109%28F07%29:Module_1&diff=127143&oldid=prev Nkuldell at 19:56, 2 July 2007 2007-07-02T19:56:34Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:56, 2 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 15:</td> <td colspan="2" class="diff-lineno">Line 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07):Start-up genome engineering | Module 1 Day 1: Start-up genome engineering]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(F07):Start-up genome engineering | Module 1 Day 1: Start-up genome engineering]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[20.109(<del class="diffchange diffchange-inline">S07</del>): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[20.109(<ins class="diffchange diffchange-inline">F07</ins>): Agarose gel electrophoresis| Module 1 Day 2: Agarose gel electrophoresis]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): DNA ligation and bacterial transformation| Module 1 Day 3: DNA ligation and bacterial transformation]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): Examine candidate clones| Module 1 Day 4: Examine candidate clones]]<br></div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 22:</td> <td colspan="2" class="diff-lineno">Line 22:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109%28S07%29:_Genome_engineering_essay]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109%28S07%29:_Genome_engineering_essay]]<br></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109:Module 1:RefactorM13| M13 refactoring workpage]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>direct link to [[20.109:Module 1:RefactorM13| M13 refactoring workpage]]<br></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">direct link to [http://parts.mit.edu/r/parts/partsdb/part_info.cgi?part_name=BBa_M1307| hard info for BBa_M1307]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): TA's notes for module 1| TA notes, mod 1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[20.109(S07): TA's notes for module 1| TA notes, mod 1]]</div></td></tr>  </table>

Nkuldell