20.109(F07):Module 3

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“Invention” is a wonderful word, derived from words meaning, “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13 and its bacterial hostIn one experiment,  we will revisit the host/phage interface and try to build a simpler one, infecting a strain bearing a minimal bacterial genome using a refactored phage genome. In a second experiment, we will use a very slightly modified phage, presenting four additional glutamic acids on the major coat protein p8, to build a nanowire that we’ll visualize on the transmission electron microscope. In a final experiment we will let the phage themselves do the building, watching them self-assemble on a polymer-surface, visualizing their structures with an atomic force microscope. Drawing on a rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel and useful materials.  
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“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13.  We will use a very slightly modified phage to build Iridium nanowires that we’ll visualize on the transmission electron microscope. Then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.  
[[Image:Macintosh HD-Users-nkuldell-Desktop-Mod4 coverartS07.png|thumb|400px|center| TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam]]
[[Image:Macintosh HD-Users-nkuldell-Desktop-Mod4 coverartS07.png|thumb|400px|center| TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam]]
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[[20.109(S07): Growth of phage materials| Module 4 Day 1: growth of phage materials]] <br>
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[[20.109(F07): Growth of phage materials| Module 3 Day 1: growth of phage materials]] <br>
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[[20.109(S07): Testing redesigned genomes| Module 4 Day 2: testing redesigned genomes]]<br>
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[[20.109(F07): Phage nanowires| Module 3 Day 2: making nanowires]]<br>
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[[20.109(S07): Building phage nanowires| Module 4 Day 3: building phage nanowires]]<br>
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[[20.109(F07): Transmission electron microscopy| Module 4 Day 3: TEM]]<br>
[[20.109 (S07): Transmission electron microscopy| Module 4 Day 4: Transmission Electron Microscopy (TEM)]] <br>
[[20.109 (S07): Transmission electron microscopy| Module 4 Day 4: Transmission Electron Microscopy (TEM)]] <br>
[[20.109 (S07): Atomic force microscopy| Module 4 Day 5: Atomic Force Microscopy (AFM)]]<br>
[[20.109 (S07): Atomic force microscopy| Module 4 Day 5: Atomic Force Microscopy (AFM)]]<br>

Revision as of 10:33, 12 July 2007

20.109(F07): Laboratory Fundamentals of Biological Engineering

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Module 3

Instructors: Angela Belcher, Natalie Kuldell and Agi Stachowiak

TA:


“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13. We will use a very slightly modified phage to build Iridium nanowires that we’ll visualize on the transmission electron microscope. Then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.


TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam
TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam

Module 3 Day 1: growth of phage materials
Module 3 Day 2: making nanowires
Module 4 Day 3: TEM
Module 4 Day 4: Transmission Electron Microscopy (TEM)
Module 4 Day 5: Atomic Force Microscopy (AFM)
Module 4 Day 6: student presentations
Module 3 Day 8: oral presentations

TA notes, mod 3
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