20.109(F07):Module 3

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (17:16, 12 October 2007) (view source)
 
(9 intermediate revisions not shown.)
Line 5: Line 5:
==Module 3==
==Module 3==
-
'''Instructors:''' [[Angela Belcher]], [[Natalie Kuldell]] and [[User:AgiStachowiak| Agi Stachowiak]]
+
'''Instructors:''' [[User:Abelcher| Angela Belcher]], [[Natalie Kuldell]] and [[User:AgiStachowiak| Agi Stachowiak]]
-
'''TA:'''  
+
'''TA:'''[[User:Barbero|Robbie Barbero]]
-
“Invention” is a wonderful word, derived from words meaning, “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13 and its bacterial hostIn one experiment,  we will revisit the host/phage interface and try to build a simpler one, infecting a strain bearing a minimal bacterial genome using a refactored phage genome. In a second experiment, we will use a very slightly modified phage, presenting four additional glutamic acids on the major coat protein p8, to build a nanowire that we’ll visualize on the transmission electron microscope. In a final experiment we will let the phage themselves do the building, watching them self-assemble on a polymer-surface, visualizing their structures with an atomic force microscope. Drawing on a rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel and useful materials.  
+
“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13.  We will use a very slightly modified phage to build Iridium nanowires that we’ll visualize on the transmission electron microscope. Then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.  
-
 
+
[[Image:Macintosh HD-Users-nkuldell-Desktop-Mod4 coverartS07.png|thumb|400px|center| TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam]]
[[Image:Macintosh HD-Users-nkuldell-Desktop-Mod4 coverartS07.png|thumb|400px|center| TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam]]
-
[[20.109(S07): Growth of phage materials| Module 4 Day 1: growth of phage materials]] <br>
+
[[20.109(F07): Growth of phage materials| Module 3 Day 1: growth of phage materials]] <br>
-
[[20.109(S07): Testing redesigned genomes| Module 4 Day 2: testing redesigned genomes]]<br>
+
[[20.109(F07): Phage nanowires| Module 3 Day 2: making nanowires]]<br>
-
[[20.109(S07): Building phage nanowires| Module 4 Day 3: building phage nanowires]]<br>
+
[[20.109(F07): Transmission electron microscopy| Module 3 Day 3: TEM]]<br>
-
[[20.109 (S07): Transmission electron microscopy| Module 4 Day 4: Transmission Electron Microscopy (TEM)]] <br>
+
[[20.109 (F07): Phage by design| Module 3 Day 4: Phage by design]] <br>
-
[[20.109 (S07): Atomic force microscopy| Module 4 Day 5: Atomic Force Microscopy (AFM)]]<br>
+
[[20.109(F07): Phage by design, pt2 | Module 3 Day 5: Phage by design, pt 2 ]]<br>
-
[[20.109 (S07): Research proposal, module 4| Module 4 Day 6: student presentations]]<br>
+
[[20.109(F07): ECD assembly| Module 3 Day 6: ECD assembly]]<br>
-
[[20.109(F07): Module 3 oral presentations| Module 3 Day 8: oral presentations]]
+
[[20.109(F07): Module 3 oral presentations| Module 3 Day 7: oral presentations]]<br>
 +
direct link to [[20.109(F07): ECD experimental variations| experimental variations]] page
[[20.109(F07): TA's notes for module 3| TA notes, mod 3]]
[[20.109(F07): TA's notes for module 3| TA notes, mod 3]]

Current revision

20.109(F07): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Fall 2007        Assignments        Lab Basics        OWW Basics       
Genome Engineering        Expression Engineering        Biomaterials Engineering              

Module 3

Instructors: Angela Belcher, Natalie Kuldell and Agi Stachowiak

TA:Robbie Barbero


“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13. We will use a very slightly modified phage to build Iridium nanowires that we’ll visualize on the transmission electron microscope. Then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.

TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam
TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam

Module 3 Day 1: growth of phage materials
Module 3 Day 2: making nanowires
Module 3 Day 3: TEM
Module 3 Day 4: Phage by design
Module 3 Day 5: Phage by design, pt 2
Module 3 Day 6: ECD assembly
Module 3 Day 7: oral presentations
direct link to experimental variations page

TA notes, mod 3
Personal tools