20.109(F07): ECD experimental variations

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| used WFblue's phage(3.9e7)
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| 40/45
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|2hr, RT
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|Part of the carbon slip tore, majority of slide did not have any IrO3. Along edges found evidence of iridium nanoparticles of fairly uniform size, as well as some nanowires. We also observed lines that may be unconjugated phage in parts of the slide.
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Revision as of 20:39, 25 November 2007

20.109(F07): Laboratory Fundamentals of Biological Engineering

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Genome Engineering        Expression Engineering        Biomaterials Engineering              

Since you are the first class to build electrochromic devices with bacteriophage, there are many variations to the protocols that may improve the biomaterials produced. This page should be used to document the experimental choices you've made along the way and their outcome. The collected wisdom that results will be used to make the experiment more robust for future researchers and may lead to alternative methods that could be explored. Thanks for doing experiments that haven't been done before and for letting everyone know how they work.

T/R lab

team name Iridium Chloride pH PFU/ul reacted with Iridium number of electrical pulses time, temp, shaking for growing nanowires comments on TEM data
TRpink 7.00 3x10^11 PFU/mL 35 2 hrs, room temp
TRyellow
TRblue
TRpurple 7.45
TRred 7.44 2.4E8 30 2hrs, rm temp
TRgreen 7.09 45 2hrs, room temp

W/F lab

team name Iridium Chloride pH PFU/ul reacted with Iridium number of electrical pulses time, temp, shaking for growing nanowires comments on TEM data
WFpink
WFyellow
WFblue 7.22 3.9*10^7
WFpurple 7.38 used WFblue's phage(3.9e7) 40/45 2hr, RT Part of the carbon slip tore, majority of slide did not have any IrO3. Along edges found evidence of iridium nanoparticles of fairly uniform size, as well as some nanowires. We also observed lines that may be unconjugated phage in parts of the slide.
WFred 7.00 2.8e6 (used 80ul instead of 40ul) 35 2hr, RT
WFgreen 7.1 7.6e6 (used 53μL to increase ratio) 45/50 1hr 54min, room T
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