20.109(F12): Mod 3 Day 2 Phage nanowires - Revision history

Jacqueline Ohmura: /* Part 1: React SWNTs:phage with Ti(I-pro)4 */

2012-11-16T19:51:03Z

Part 1: React SWNTs:phage with Ti(I-pro)4

←Older revision		Revision as of 19:51, 16 November 2012
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	#* a. If your phage volume is less than 250 uL total. You may simply use 5 ml of 95% ethanol.		#* a. If your phage volume is less than 250 uL total. You may simply use 5 ml of 95% ethanol.
	#* b. If your phage volume is > 250 uL total, calculate how much 100% ethanol you would need to add to achieve a 95% solution. Assume that your phage solution is 100% water.		#* b. If your phage volume is > 250 uL total, calculate how much 100% ethanol you would need to add to achieve a 95% solution. Assume that your phage solution is 100% water.
-	# Add ~~this~~ volume (from a. or b.) to a 250 mL erlenmyer flask with a stir bar and set aside.	+	# Add ethanol volume from a. or b. without phage to a 250 mL erlenmyer flask with a stir bar and set aside.
	# Prepare a super-cooled bath:		# Prepare a super-cooled bath:
	#* Mix ethylene glycol and ethanol in a 1:1 volume ratio (make up a total of 100 mL).		#* Mix ethylene glycol and ethanol in a 1:1 volume ratio (make up a total of 100 mL).
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	# Add your complexed phage solution to the EtOH:titanium and stir vigorously for 20 minutes.		# Add your complexed phage solution to the EtOH:titanium and stir vigorously for 20 minutes.
	# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution should turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.		# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution should turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.
-
-

	===Part 2: Prepare a grid for TEM===		===Part 2: Prepare a grid for TEM===

Shannon K. Alford at 20:39, 15 November 2012

2012-11-15T20:39:54Z

←Older revision		Revision as of 20:39, 15 November 2012
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	# Use the markings on the tube to estimate the volume of the nanowires.		# Use the markings on the tube to estimate the volume of the nanowires.
	# Vortex the nanowires if they look clumpy.		# Vortex the nanowires if they look clumpy.
		+
	<br>		<br>
	[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]		[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]
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	For all groups:		For all groups:
	# Chill your complexed phage on ice on your bench until you are ready to react it with the titanium.		# Chill your complexed phage on ice on your bench until you are ready to react it with the titanium.
-	# Calculate the amount of material necessary for making TiO2 Nano composites. ~~Assuming that the~~ volume of phage is ~~100% water~~, calculate ~~the amount of pure~~ ethanol to add to ~~the phage which will result in~~ a 95% ~~ethanol~~ solution. ~~Additionally, calculate how much Ti~~(~~I-pro~~)~~4 you must add~~ to ~~your phage in order to achieve~~ a 15:1 Ti(I-pro)4:phage ratio*.	+	# Calculate the amount of material necessary for making TiO2 Nano composites. The reaction should take place in a 95% ethanol solution. You have two choices:
-	# Prepare a bath	+	#* a. If your phage volume is less than 250 uL total. You may simply use 5 ml of 95% ethanol.
-	#* Mix ethylene glycol and ethanol in a 1:1 volume ratio.	+	#* b. If your phage volume is > 250 uL total, calculate how much 100% ethanol you would need to add to achieve a 95% solution. Assume that your phage solution is 100% water.
-	#* Place a 250ml Erlenmeyer flask inside an evaporation dish ~~and fill~~ the ~~flask with the amount of ethanol calculated in step 2. Add~~ a stir ~~bar~~.	+	# Add this volume (from a. or b.) to a 250 mL erlenmyer flask with a stir bar and set aside.
		+	# Prepare a super-cooled bath:
		+	#* Mix ethylene glycol and ethanol in a 1:1 volume ratio (make up a total of 100 mL).
		+	#* Place the 250ml Erlenmeyer flask with your ethanol solution + stir bar inside an evaporation dish in the fume hood on a stir plate.
	#* Fill the evaporation dish with the ethylene glycol and ethanol mixture match the liquid level inside the Erlenmeyer flask.		#* Fill the evaporation dish with the ethylene glycol and ethanol mixture match the liquid level inside the Erlenmeyer flask.
	#* Add ~10 dry ice chunks to the evaporation dish and let sit for 10 min. You can measure the temperature of this mixture. It should measure ~ negative 40°C.		#* Add ~10 dry ice chunks to the evaporation dish and let sit for 10 min. You can measure the temperature of this mixture. It should measure ~ negative 40°C.
-	# ~~Place~~ the ~~dish and~~ the ~~jar with~~ the ~~EtOH~~ in the ~~fume hood on a stir plate~~.	+	# Next, calculate how much Ti(I-pro)4 you must add to your phage in order to achieve a 15:1 TiO2:phage ratio. The '''15:1 gram ratio''' is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2.
-	# Put on lab coat, gloves, and safety glasses for this next step. Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.	+	# Quantities you need to know:
		+	#* MW of phage = 1.8 x 10^7 g/mol
		+	#* MW of Ti(I-pro)4 = 284.22 g/mol
		+	#* MW of TiO2 = 80 g/mol
		+	#* Concentration of Ti(I-pro)4 = 0.96 g/mL
		+	Think about the following progression:
		+	phage particle # --> moles phage --> weight phage --> weight TiO2 --> moles TiO2 =
		+	moles Ti(I-pro)4 --> volume of Ti(I-pro)4 to add
		+	# [[Put on lab coat, gloves, and safety glasses for this next step.]] Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.
	# Add your complexed phage solution to the EtOH:titanium and stir vigorously for 20 minutes.		# Add your complexed phage solution to the EtOH:titanium and stir vigorously for 20 minutes.
-	# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution ~~whould~~ turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.	+	# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution should turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.

-	*The 15:1 gram ratio is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2 <br>	+

	===Part 2: Prepare a grid for TEM===		===Part 2: Prepare a grid for TEM===

Nkuldell: /* Protocols */

2012-11-15T17:39:50Z

Protocols

←Older revision		Revision as of 17:39, 15 November 2012
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	# Hand the pellet of phage:SWNTs:TiO2 to the teaching faculty who will begin to prepare the paste needed to build them into the solar cell.		# Hand the pellet of phage:SWNTs:TiO2 to the teaching faculty who will begin to prepare the paste needed to build them into the solar cell.

-	===Part 3: Research pre-proposal===	+	===Part 4: Research pre-proposal===
	Pre-proposals will be due in lecture on M3D4 (11.29.12) at 11AM. That's coming up soon! Use your time today to look carefully at the requirements for this pre-proposal assignment. The requirements are posted [[20.109(F12): Written research pre-proposal\| here.]] Today you and your lab partner should work through the research topics you identified as interesting and decide on one (or at most two) that are worth researching more. <br>		Pre-proposals will be due in lecture on M3D4 (11.29.12) at 11AM. That's coming up soon! Use your time today to look carefully at the requirements for this pre-proposal assignment. The requirements are posted [[20.109(F12): Written research pre-proposal\| here.]] Today you and your lab partner should work through the research topics you identified as interesting and decide on one (or at most two) that are worth researching more. <br>
	DONE!		DONE!

Jacqueline Ohmura: /* Phage:SWNTs:TiO2 & Phage:gold:TiO2 nanocomposite */

2012-11-15T17:37:12Z

Phage:SWNTs:TiO2 & Phage:gold:TiO2 nanocomposite

←Older revision		Revision as of 17:37, 15 November 2012
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	{{Template:20.109(F12)}}		{{Template:20.109(F12)}}
	<div style="padding: 10px; width: 640px; border: 5px solid #009966;">		<div style="padding: 10px; width: 640px; border: 5px solid #009966;">
-	=<center>Phage:SWNTs:TiO2 & Phage:gold:TiO2 ~~nanocomposite~~</center>=	+	=<center>Phage:SWNTs:TiO2 & Phage:gold:TiO2 nanocomposites</center>=
	==Introduction==		==Introduction==
	Eventually, we'll build a solar cell where the photoanode is made from the material that we're synthesizing today. By templating the assembly of the nanotubes-TiO2 nanocrystal with the M13 phage, the electronic properties of the photoanode are improved.		Eventually, we'll build a solar cell where the photoanode is made from the material that we're synthesizing today. By templating the assembly of the nanotubes-TiO2 nanocrystal with the M13 phage, the electronic properties of the photoanode are improved.

Jacqueline Ohmura: /* Phage:SWNTs:TiO2 nanocomposite */

2012-11-15T17:36:57Z

Phage:SWNTs:TiO2 nanocomposite

←Older revision		Revision as of 17:36, 15 November 2012
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	{{Template:20.109(F12)}}		{{Template:20.109(F12)}}
	<div style="padding: 10px; width: 640px; border: 5px solid #009966;">		<div style="padding: 10px; width: 640px; border: 5px solid #009966;">
-	=<center>Phage:SWNTs:TiO2 nanocomposite</center>=	+	=<center>Phage:SWNTs:TiO2 & Phage:gold:TiO2 nanocomposite</center>=
	==Introduction==		==Introduction==
	Eventually, we'll build a solar cell where the photoanode is made from the material that we're synthesizing today. By templating the assembly of the nanotubes-TiO2 nanocrystal with the M13 phage, the electronic properties of the photoanode are improved.		Eventually, we'll build a solar cell where the photoanode is made from the material that we're synthesizing today. By templating the assembly of the nanotubes-TiO2 nanocrystal with the M13 phage, the electronic properties of the photoanode are improved.

Nkuldell: /* Protocols */

2012-11-15T03:39:43Z

Protocols

←Older revision		Revision as of 03:39, 15 November 2012
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	# Calculate the amount of material necessary for making TiO2 Nano composites. Assuming that the volume of phage is 100% water, calculate the amount of pure ethanol to add to the phage which will result in a 95% ethanol solution. Additionally, calculate how much Ti(I-pro)4 you must add to your phage in order to achieve a 15:1 Ti(I-pro)4:phage ratio*.		# Calculate the amount of material necessary for making TiO2 Nano composites. Assuming that the volume of phage is 100% water, calculate the amount of pure ethanol to add to the phage which will result in a 95% ethanol solution. Additionally, calculate how much Ti(I-pro)4 you must add to your phage in order to achieve a 15:1 Ti(I-pro)4:phage ratio*.
	# Prepare a bath		# Prepare a bath
-	## Mix ethylene glycol and ethanol in a 1:1 volume ratio.	+	#* Mix ethylene glycol and ethanol in a 1:1 volume ratio.
-	## Place a 250ml Erlenmeyer flask inside an evaporation dish and fill the flask with the amount of ethanol calculated in step 2. Add a stir bar.	+	#* Place a 250ml Erlenmeyer flask inside an evaporation dish and fill the flask with the amount of ethanol calculated in step 2. Add a stir bar.
-	## Fill the evaporation dish with the ethylene glycol and ethanol mixture match the liquid level inside the Erlenmeyer flask.	+	#* Fill the evaporation dish with the ethylene glycol and ethanol mixture match the liquid level inside the Erlenmeyer flask.
-	## Add ~10 dry ice chunks to the evaporation dish and let sit for 10 min. You can measure the temperature of this mixture. It should measure ~ negative 40°C.	+	#* Add ~10 dry ice chunks to the evaporation dish and let sit for 10 min. You can measure the temperature of this mixture. It should measure ~ negative 40°C.
	# Place the dish and the jar with the EtOH in the fume hood on a stir plate.		# Place the dish and the jar with the EtOH in the fume hood on a stir plate.
	# Put on lab coat, gloves, and safety glasses for this next step. Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.		# Put on lab coat, gloves, and safety glasses for this next step. Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.
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	# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution whould turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.		# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution whould turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.

-	*The 15:1 gram ratio is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2	+	*The 15:1 gram ratio is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2 <br>
-	<br>	+

	===Part 2: Prepare a grid for TEM===		===Part 2: Prepare a grid for TEM===

Jacqueline Ohmura: /* Part 1: React SWNTs:phage with Ti(I-pro)4 */

2012-11-15T02:38:32Z

Part 1: React SWNTs:phage with Ti(I-pro)4

←Older revision		Revision as of 02:38, 15 November 2012
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	# Place the dish and the jar with the EtOH in the fume hood on a stir plate.		# Place the dish and the jar with the EtOH in the fume hood on a stir plate.
	# Put on lab coat, gloves, and safety glasses for this next step. Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.		# Put on lab coat, gloves, and safety glasses for this next step. Add the calculated amount of Ti(I-pro)4 to the supercooled EtOH. Stir at least 5 minutes.
-	# Add your complexed phage solution to the EtOH:titanium and stir vigorously for ~~3 hours~~.	+	# Add your complexed phage solution to the EtOH:titanium and stir vigorously for 20 minutes.
-		+	# Remove the Erlenmeyer flask from the bath and place it back on the stir plate. Leave the mixture on the plate until it reaches room temperature. After a maximum of an hour your solution whould turn grey (SWNT complexed phage) or white (gold complexed phage) indicating that the reaction has completed.
		+
	*The 15:1 gram ratio is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2		*The 15:1 gram ratio is based off of a calculation of the phage surface area to the surface area of the phage covered in TiO2, as the goal is to cover the phage completely with TiO2
	<br>		<br>

Jacqueline Ohmura: /* Part 1: React SWNTs:phage with Ti(I-pro)4 */

2012-11-13T04:12:01Z

Part 1: React SWNTs:phage with Ti(I-pro)4

←Older revision		Revision as of 04:12, 13 November 2012
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	===Part 1: React SWNTs:phage with Ti(I-pro)4===		===Part 1: React SWNTs:phage with Ti(I-pro)4===
	Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're at the chemical hood. The reaction of the complexed phage with the titanium will take place in the hood at supercooled temperatures (a bath at ~ -40°C). Once the titanium has been deposited on the surface of the phage, the solution is less hazardous, though you should still treat the materials with care since no reactions run to completion		Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're at the chemical hood. The reaction of the complexed phage with the titanium will take place in the hood at supercooled temperatures (a bath at ~ -40°C). Once the titanium has been deposited on the surface of the phage, the solution is less hazardous, though you should still treat the materials with care since no reactions run to completion
-	~~<br>~~
-	~~[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]~~

	For SWNT phage only:		For SWNT phage only:
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	# Use the markings on the tube to estimate the volume of the nanowires.		# Use the markings on the tube to estimate the volume of the nanowires.
	# Vortex the nanowires if they look clumpy.		# Vortex the nanowires if they look clumpy.
		+	<br>
		+	[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]

	For all groups:		For all groups:

Jacqueline Ohmura: /* Part 1: React SWNTs:phage with Ti(I-pro)4 */

2012-11-13T04:11:36Z

Part 1: React SWNTs:phage with Ti(I-pro)4

←Older revision		Revision as of 04:11, 13 November 2012
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	<br>		<br>
	[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]		[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]
-	~~<br>~~
-	~~[[Image:Salt ice bath.jpg\|thumb\| 200px\| ice+salt bath]][[Image:ReactionOnIce.jpg\|thumb\|200px\| distillation jar, chillin']][[Image:Phage SWNTs TiO2reaction.jpg\|thumb\|200px\| reactions done!]]<br>~~

	For SWNT phage only:		For SWNT phage only:

Jacqueline Ohmura: /* Part 1: React SWNTs:phage with Ti(I-pro)4 */

2012-11-13T04:11:18Z

Part 1: React SWNTs:phage with Ti(I-pro)4

←Older revision		Revision as of 04:11, 13 November 2012
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	Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're at the chemical hood. The reaction of the complexed phage with the titanium will take place in the hood at supercooled temperatures (a bath at ~ -40°C). Once the titanium has been deposited on the surface of the phage, the solution is less hazardous, though you should still treat the materials with care since no reactions run to completion		Today's lab has some safety hazards and you must work extremely carefully. Lab coats, gloves and goggles are a must when you're at the chemical hood. The reaction of the complexed phage with the titanium will take place in the hood at supercooled temperatures (a bath at ~ -40°C). Once the titanium has been deposited on the surface of the phage, the solution is less hazardous, though you should still treat the materials with care since no reactions run to completion
	<br>		<br>
-	[[Image:Labex.jpg\|thumb\|200px]]	+	[[Image:Labex.jpg\|thumb\|200px\|Bath set-up]]
	<br>		<br>
	[[Image:Salt ice bath.jpg\|thumb\| 200px\| ice+salt bath]][[Image:ReactionOnIce.jpg\|thumb\|200px\| distillation jar, chillin']][[Image:Phage SWNTs TiO2reaction.jpg\|thumb\|200px\| reactions done!]]<br>		[[Image:Salt ice bath.jpg\|thumb\| 200px\| ice+salt bath]][[Image:ReactionOnIce.jpg\|thumb\|200px\| distillation jar, chillin']][[Image:Phage SWNTs TiO2reaction.jpg\|thumb\|200px\| reactions done!]]<br>