20.109(F12): Quick Facts

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(Taq Polymerase)
(Taq Polymerase)
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* Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract].
* Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract].
** So would you use Taq for cloning purposes?
** So would you use Taq for cloning purposes?
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** What is the probability of finding a mutation in a GFP gene?
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** What is the probability of finding a mutation in a GFP gene PCR-ed up using Taq polymerase?

Revision as of 15:09, 8 September 2012

DNA Polymerases

Taq Polymerase

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