20.109(F12): Quick Facts

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(Taq Polymerase)
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* If you had a plasmid of size 3 kb, at a concentration typical of high copy plasmids (~150 ng/µL), and you added 1 µL of DNA into a PCR reaction (50 µL), given the typical conditions for a PCR, what are the concentrations of plasmid and primers respectively?
* If you had a plasmid of size 3 kb, at a concentration typical of high copy plasmids (~150 ng/µL), and you added 1 µL of DNA into a PCR reaction (50 µL), given the typical conditions for a PCR, what are the concentrations of plasmid and primers respectively?
* Would the concentrations be sufficient to explain why primers preferentially bind to the template compared to re-annealing of the template? If so, why? If not, why?
* Would the concentrations be sufficient to explain why primers preferentially bind to the template compared to re-annealing of the template? If so, why? If not, why?
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P.S. to all the 20.109 students, these are questions that have gone through my head, but I haven't written down an answer for. Lemme know if you've got an answer!*'''[[User:Eric Ma|Eric Ma]] 16:47, 8 September 2012 (EDT)''':

Revision as of 16:47, 8 September 2012

DNA Polymerases

Taq Polymerase

  • If you had a plasmid of size 3 kb, at a concentration typical of high copy plasmids (~150 ng/µL), and you added 1 µL of DNA into a PCR reaction (50 µL), given the typical conditions for a PCR, what are the concentrations of plasmid and primers respectively?
  • Would the concentrations be sufficient to explain why primers preferentially bind to the template compared to re-annealing of the template? If so, why? If not, why?

P.S. to all the 20.109 students, these are questions that have gone through my head, but I haven't written down an answer for. Lemme know if you've got an answer!*Eric Ma 16:47, 8 September 2012 (EDT):

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