20.109(S08): Spring 2008 schedule

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| PDF of slides is [[Media:BE109_S08_Mod1-Day2_ENGELWARD.pdf|here]]
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
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Revision as of 23:57, 12 February 2008

20.109(S08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 5 ANS Orientation Slides are here (warning: .ppt download)
1 1 Thur Feb 7 BE Day 1: DNA engineering using PCR PDF of slides is here
1 2 Tue Feb 12 BE PDF of slides is here Day 2: Clean and cut DNA
1 3 Thur Feb 14 BE Day 3: Agarose gel electrophoresis
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur Feb 21 BE Day 4: DNA ligation and bacterial transformation
1 5 Tue Feb 26 BE Day 5: Examine candidate clones
1 6 Thur Feb 28 BE Day 6: Restriction map and tissue culture
1 7 Tue Mar 4 BE Day 7: Lipofection
1 8 Thur Mar 6 BE Day 8: FACS analysis
2 1 Tue Mar 11 AJ Day 1: Start-up protein engineering
2 2 Thur Mar 13 AJ Day 2: Site-directed mutagenesis
2 3 Tue Mar 18 AJ Day 3: Prepare expression system
2 4 Thur Mar 20 AJ Day 4: Induce protein expression
Spring Break Mar 24-28 No lab all week
2 5 Tue Apr 1 AJ Day 5: Characterize protein expression
2 6 Thur Apr 3 AJ Day 6: Assay protein behavior
2 7 Tue Apr 8 AJ Day 7: Data analysis
2 8 Thur Apr 10 AJ Day 8: Student presentations
3 1 Tues Apr 15 ANS Day 1: Start-up biomaterials engineering
3 2 Thur Apr 17 ANS Day 2: Initiate cell culture
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 3 Thur Apr 24 ANS Module 3 Day 3: Testing cell viability
3 4 Tue Apr 29 ANS Module 3 Day 4: Preparing cells for analysis
3 5 Thur May 1 ANS Module 3 Day 5: Transcript-level analysis
3 6 Tue May 6 ANS Module 3 Day 6: Protein-level analysis
3 7 Thur May 8 ANS Module 3 Day 7: Wrap-up analysis
3 8 Tue May 13 ANS Module 3 Day 8: Student presentations
Thur May 15 No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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