20.109(S08): Spring 2008 schedule

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| Tue Mar 11
| Tue Mar 11
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]]
| [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]]
|
|
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| Thur Mar 13
| Thur Mar 13
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]]
| [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]]
|
|
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| Tue Mar 18  
| Tue Mar 18  
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Prepare expression system (Day3) | Day 3: Prepare expression system]]
| [[20.109(S08):Prepare expression system (Day3) | Day 3: Prepare expression system]]
|
|
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| Thur Mar 20
| Thur Mar 20
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Induce protein expression (Day4) | Day 4: Induce protein expression]]
| [[20.109(S08):Induce protein expression (Day4) | Day 4: Induce protein expression]]
|
|
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| Tue Apr 1
| Tue Apr 1
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Characterize protein expression (Day5) | Day 5: Characterize protein expression]]
| [[20.109(S08):Characterize protein expression (Day5) | Day 5: Characterize protein expression]]
|   
|   
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| Thur Apr 3
| Thur Apr 3
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Assay protein behavior (Day6) | Day 6: Assay protein behavior]]
| [[20.109(S08):Assay protein behavior (Day6) | Day 6: Assay protein behavior]]
|  
|  
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| Tue Apr 8  
| Tue Apr 8  
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Data analysis (Day7) | Day 7: Data analysis]]
| [[20.109(S08):Data analysis (Day7) | Day 7: Data analysis]]
|  
|  
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| Thur Apr 10  
| Thur Apr 10  
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Student presentations (M2D8)| Day 8: Student presentations]]<br>
| [[20.109(S08):Student presentations (M2D8)| Day 8: Student presentations]]<br>
|
|
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| Tues Apr 15
| Tues Apr 15
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Start-up biomaterials engineering (Day1)| Day 1: Start-up biomaterials engineering]]
| [[20.109(S08):Start-up biomaterials engineering (Day1)| Day 1: Start-up biomaterials engineering]]
|  
|  
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| Thur Apr 17
| Thur Apr 17
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Initiate cell culture (Day2)| Day 2: Initiate cell culture]]
| [[20.109(S08):Initiate cell culture (Day2)| Day 2: Initiate cell culture]]
|  
|  
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| Thur Apr 24
| Thur Apr 24
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Testing cell viability (Day3)| Module 3 Day 3: Testing cell viability]]
| [[20.109(S08):Testing cell viability (Day3)| Module 3 Day 3: Testing cell viability]]
|  
|  
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| Tue Apr 29  
| Tue Apr 29  
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Preparing cells for analysis (Day4)| Module 3 Day 4: Preparing cells for analysis]]
| [[20.109(S08):Preparing cells for analysis (Day4)| Module 3 Day 4: Preparing cells for analysis]]
|  
|  
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| Thur May 1
| Thur May 1
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
|  [[20.109(S08):Transcript-level analysis (Day5)| Module 3 Day 5: Transcript-level analysis]]
|  [[20.109(S08):Transcript-level analysis (Day5)| Module 3 Day 5: Transcript-level analysis]]
|  
|  
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| Tue May 6
| Tue May 6
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Protein-level analysis (Day6)| Module 3 Day 6: Protein-level analysis]]
| [[20.109(S08):Protein-level analysis (Day6)| Module 3 Day 6: Protein-level analysis]]
|  
|  
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| Thur May 8
| Thur May 8
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
| [[20.109(S08):Wrap-up analysis (Day7)| Module 3 Day 7: Wrap-up analysis]]  
| [[20.109(S08):Wrap-up analysis (Day7)| Module 3 Day 7: Wrap-up analysis]]  
|  
|  
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| Tue May 13  
| Tue May 13  
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
|[[20.109(S08):Student presentations (Day8)| Module 3 Day 8: Student presentations]]
|[[20.109(S08):Student presentations (Day8)| Module 3 Day 8: Student presentations]]
|  
|  

Revision as of 00:01, 13 February 2008

20.109(S08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 5 ANS VL, BE Orientation Slides are here (warning: .ppt download)
1 1 Thur Feb 7 BE DW Day 1: DNA engineering using PCR PDF of slides is here
1 2 Tue Feb 12 BE DW Day 2: Clean and cut DNA PDF of slides is here
1 3 Thur Feb 14 BE DW Day 3: Agarose gel electrophoresis
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur Feb 21 BE DW Day 4: DNA ligation and bacterial transformation
1 5 Tue Feb 26 BE DW Day 5: Examine candidate clones
1 6 Thur Feb 28 BE DW Day 6: Restriction map and tissue culture
1 7 Tue Mar 4 BE DW Day 7: Lipofection
1 8 Thur Mar 6 BE DW Day 8: FACS analysis
2 1 Tue Mar 11 AJ VL Day 1: Start-up protein engineering
2 2 Thur Mar 13 AJ VL Day 2: Site-directed mutagenesis
2 3 Tue Mar 18 AJ VL Day 3: Prepare expression system
2 4 Thur Mar 20 AJ VL Day 4: Induce protein expression
Spring Break Mar 24-28 No lab all week
2 5 Tue Apr 1 AJ VL Day 5: Characterize protein expression
2 6 Thur Apr 3 AJ VL Day 6: Assay protein behavior
2 7 Tue Apr 8 AJ VL Day 7: Data analysis
2 8 Thur Apr 10 AJ VL Day 8: Student presentations
3 1 Tues Apr 15 ANS BE Day 1: Start-up biomaterials engineering
3 2 Thur Apr 17 ANS BE Day 2: Initiate cell culture
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 3 Thur Apr 24 ANS BE Module 3 Day 3: Testing cell viability
3 4 Tue Apr 29 ANS BE Module 3 Day 4: Preparing cells for analysis
3 5 Thur May 1 ANS BE Module 3 Day 5: Transcript-level analysis
3 6 Tue May 6 ANS BE Module 3 Day 6: Protein-level analysis
3 7 Thur May 8 ANS BE Module 3 Day 7: Wrap-up analysis
3 8 Tue May 13 ANS BE Module 3 Day 8: Student presentations
Thur May 15 No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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