20.109(S08): Spring 2008 schedule

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Revision as of 16:28, 30 April 2008

20.109(S08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 5 ANS VL, BE Orientation Slides are here (warning: .ppt download)
1 1 Thur Feb 7 BE DW Day 1: DNA engineering using PCR PDF of slides is here
1 2 Tue Feb 12 BE DW Day 2: Clean and cut DNA PDF of slides is here
1 3 Thur Feb 14 BE DW Day 3: Agarose gel electrophoresis PDF of slides is here
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur Feb 21 BE DW Day 4: DNA ligation and bacterial transformation PDF of slides is here
1 5 Tue Feb 26 BE DW Day 5: Examine candidate clones PDF of slides is here
1 6 Thur Feb 28 BE DW Day 6: Restriction map and tissue culture PDF of slides is here
1 7 Tue Mar 4 BE DW Day 7: Lipofection PDF of slides is here
1 8 Thur Mar 6 BE DW Day 8: FACS analysis PDF of slides is here
2 1 Tue Mar 11 AJ VL Day 1: Start-up protein engineering 'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.)
2 2 Thur Mar 13 AJ VL Day 2: Site-directed mutagenesis For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary.
2 3 Tue Mar 18 AJ VL Day 3: Prepare expression system For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary.
2 4 Thur Mar 20 AJ VL Day 4: Induce protein expression For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary.
Spring Break Mar 24-28 No lab all week
2 5 Tue Apr 1 AJ VL Day 5: Characterize protein expression For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary.
2 6 Thur Apr 3 AJ VL Day 6: Assay protein behavior Please see Attisa's user page
2 7 Tue Apr 8 AJ VL Day 7: Data analysis 'High throughput screening' handout is available in class.
2 8 Thur Apr 10 AJ VL Day 8: Student presentations
'"Real-world" protein engineering' handout is available in class.
3 1 Tues Apr 15 ANS BE Day 1: Start-up biomaterials engineering PDF of slides is here
3 2 Thur Apr 17 ANS BE Day 2: Initiate cell culture PDF of slides is here
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 3 Thur Apr 24 ANS/NL BE Module 3 Day 3: Testing cell viability PDF of slides is here
3 4 Tue Apr 29 ANS BE Module 3 Day 4: Preparing cells for analysis PDF of slides is here
3 5 Thur May 1 ANS BE Module 3 Day 5: Transcript-level analysis
3 6 Tue May 6 ANS BE Module 3 Day 6: Protein-level analysis
3 7 Thur May 8 ANS BE Module 3 Day 7: Wrap-up analysis
3 8 Tue May 13 ANS BE Module 3 Day 8: Student presentations
Thur May 15 No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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