20.109(S08): Spring 2008 schedule

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|  
|  
| Tue Feb 5  
| Tue Feb 5  
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|  
+
| ANS
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|  
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| VL, BE
| [[20.109(S08):Lab tour | Orientation]]
| [[20.109(S08):Lab tour | Orientation]]
-
|
+
| Slides are [[Media:20.109_OrientationLect(S08).ppt|here]] (warning: .ppt download)
|--
|--
| colspan="7" |
| colspan="7" |
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| Thur Feb 7
| Thur Feb 7
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]]
| [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]]
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|  
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| PDF of slides is [[Media:BE109_S08_Mod1-Day1_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Tue Feb 12  
| Tue Feb 12  
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]]
-
|  
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day2_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Thur Feb 14
| Thur Feb 14
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]]
| [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]]
-
|
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day3_ENGELWARD.pdf|here]]
|--
|--
|
|
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| Thur Feb 21
| Thur Feb 21
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]]
| [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]]
-
|  
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day4_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Tue Feb 26
| Tue Feb 26
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]]
| [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]]
-
|  
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day5_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Thur Feb 28
| Thur Feb 28
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]]
| [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]]
-
|  
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day6_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Tue Mar 4  
| Tue Mar 4  
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]]
| [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]]
-
|
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day7_ENGELWARD.pdf|here]]
|--
|--
| 1
| 1
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| Thur Mar 6
| Thur Mar 6
| BE
| BE
-
|  
+
| DW
| [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]]
| [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]]
-
|
+
| PDF of slides is [[Media:BE109_S08_Mod1-Day8_ENGELWARD.pdf|here]]
|--
|--
| colspan="7" |
| colspan="7" |
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| Tue Mar 11
| Tue Mar 11
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]]
| [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]]
-
|
+
| 'Introduction to the module' handout ''hardcopies'' are available, in class. (Ditto other Mod2 lectures.)
|--
|--
| 2
| 2
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| Thur Mar 13
| Thur Mar 13
| AJ
| AJ
-
|  
+
| VL
| [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]]
| [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]]
-
|
+
| For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary.
|--
|--
| 2
| 2
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| Tue Mar 18  
| Tue Mar 18  
| AJ
| AJ
-
|  
+
| VL
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| [[20.109(S08): Prepare expression system (Day3) | Day 3: Prepare expression system]]
+
| [[20.109(S08):Prepare expression system (Day3) | Day 3: Prepare expression system]]
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|
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| For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary.
|--
|--
| 2
| 2
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| Thur Mar 20
| Thur Mar 20
| AJ
| AJ
-
|  
+
| VL
-
|  
+
| [[20.109(S08):Induce protein expression (Day4) | Day 4: Induce protein expression]]
-
|
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| For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary.
|--
|--
|
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| Tue Apr 1
| Tue Apr 1
| AJ
| AJ
-
|  
+
| VL
-
|  
+
| [[20.109(S08):Characterize protein expression (Day5) | Day 5: Characterize protein expression]]
-
|
+
| For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary.
|--
|--
| 2
| 2
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| Thur Apr 3
| Thur Apr 3
| AJ
| AJ
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|  
+
| VL
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|  
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| [[20.109(S08):Assay protein behavior (Day6) | Day 6: Assay protein behavior]]
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| Please see [[User:Atissa | Attisa's]] user page
|--
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| Tue Apr 8  
| Tue Apr 8  
| AJ
| AJ
-
|  
+
| VL
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|  
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| [[20.109(S08):Data analysis (Day7) | Day 7: Data analysis]]
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| 'High throughput screening' handout is available in class.
|--
|--
| 2
| 2
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| Thur Apr 10  
| Thur Apr 10  
| AJ
| AJ
-
|  
+
| VL
-
|  
+
| [[20.109(S08):Student presentations (M2D8)| Day 8: Student presentations]]<br>
-
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|'"Real-world" protein engineering' handout is available in class.
|--
|--
| colspan="7" |  
| colspan="7" |  
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| Tues Apr 15
| Tues Apr 15
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
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|  
+
| BE
-
|  
+
| [[20.109(S08):Start-up biomaterials engineering (Day1)| Day 1: Start-up biomaterials engineering]]
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|  
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| PDF of slides is [[Media:20.109(S08)_M3Lec1.pdf|here]]
|--
|--
| 3
| 3
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| Thur Apr 17
| Thur Apr 17
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
-
|  
+
| [[20.109(S08):Initiate cell culture (Day2)| Day 2: Initiate cell culture]]
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| PDF of slides is [[Media:20.109(S08)_M3Lec2.pdf|here]]
|--
|--
|
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| 3
| 3
| Thur Apr 24
| Thur Apr 24
-
| [[User:AgiStachowiak | ANS]]
+
| [[User:AgiStachowiak | ANS]]/NL
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|  
+
| BE
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|  
+
| [[20.109(S08):Testing cell viability (Day3)| Module 3 Day 3: Testing cell viability]]
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|  
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| PDF of slides is [[Media:20.109(S08)_M3Lec3-shrink.pdf|here]]
|--
|--
| 3
| 3
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| Tue Apr 29  
| Tue Apr 29  
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
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|  
+
| [[20.109(S08):Preparing cells for analysis (Day4)| Module 3 Day 4: Preparing cells for analysis]]
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|  
+
| PDF of slides is [[Media:20.109(S08)_M3Lec4.pdf|here]]
|--
|--
| 3
| 3
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| Thur May 1
| Thur May 1
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
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|   
+
[[20.109(S08):Transcript-level analysis (Day5)| Module 3 Day 5: Transcript-level analysis]]
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|  
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| PDF of slides is [[Media:20.109(S08)_M3Lec5-shrunk.pdf|here]]
|--
|--
| 3
| 3
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| Tue May 6
| Tue May 6
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
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|  
+
| [[20.109(S08):Protein-level analysis (Day6)| Module 3 Day 6: Protein-level analysis]]
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|  
+
| PDF of slides is [[Media:20.109(S08)_M3Lec6.pdf|here]]
|--
|--
| 3
| 3
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| Thur May 8
| Thur May 8
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
-
|  
+
| [[20.109(S08):Wrap-up analysis (Day7)| Module 3 Day 7: Wrap-up analysis]]
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|  
+
| PDF of slides is [[Media:20.109(S08)_M3Lec7-shrink.pdf|here]]
|--
|--
| 3
| 3
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| Tue May 13  
| Tue May 13  
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| BE
-
|  
+
|[[20.109(S08):Student presentations (Day8)| Module 3 Day 8: Student presentations]]
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|  
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| (Chalkboard lecture)
|--
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| Lecture only, no lab
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| No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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|}

Current revision

20.109(S08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 5 ANS VL, BE Orientation Slides are here (warning: .ppt download)
1 1 Thur Feb 7 BE DW Day 1: DNA engineering using PCR PDF of slides is here
1 2 Tue Feb 12 BE DW Day 2: Clean and cut DNA PDF of slides is here
1 3 Thur Feb 14 BE DW Day 3: Agarose gel electrophoresis PDF of slides is here
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur Feb 21 BE DW Day 4: DNA ligation and bacterial transformation PDF of slides is here
1 5 Tue Feb 26 BE DW Day 5: Examine candidate clones PDF of slides is here
1 6 Thur Feb 28 BE DW Day 6: Restriction map and tissue culture PDF of slides is here
1 7 Tue Mar 4 BE DW Day 7: Lipofection PDF of slides is here
1 8 Thur Mar 6 BE DW Day 8: FACS analysis PDF of slides is here
2 1 Tue Mar 11 AJ VL Day 1: Start-up protein engineering 'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.)
2 2 Thur Mar 13 AJ VL Day 2: Site-directed mutagenesis For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary.
2 3 Tue Mar 18 AJ VL Day 3: Prepare expression system For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary.
2 4 Thur Mar 20 AJ VL Day 4: Induce protein expression For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary.
Spring Break Mar 24-28 No lab all week
2 5 Tue Apr 1 AJ VL Day 5: Characterize protein expression For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary.
2 6 Thur Apr 3 AJ VL Day 6: Assay protein behavior Please see Attisa's user page
2 7 Tue Apr 8 AJ VL Day 7: Data analysis 'High throughput screening' handout is available in class.
2 8 Thur Apr 10 AJ VL Day 8: Student presentations
'"Real-world" protein engineering' handout is available in class.
3 1 Tues Apr 15 ANS BE Day 1: Start-up biomaterials engineering PDF of slides is here
3 2 Thur Apr 17 ANS BE Day 2: Initiate cell culture PDF of slides is here
Patriot's Day April 21-22 No lab Apr 22 or Apr 23
3 3 Thur Apr 24 ANS/NL BE Module 3 Day 3: Testing cell viability PDF of slides is here
3 4 Tue Apr 29 ANS BE Module 3 Day 4: Preparing cells for analysis PDF of slides is here
3 5 Thur May 1 ANS BE Module 3 Day 5: Transcript-level analysis PDF of slides is here
3 6 Tue May 6 ANS BE Module 3 Day 6: Protein-level analysis PDF of slides is here
3 7 Thur May 8 ANS BE Module 3 Day 7: Wrap-up analysis PDF of slides is here
3 8 Tue May 13 ANS BE Module 3 Day 8: Student presentations (Chalkboard lecture)
Thur May 15 No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
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