20.109(S11): Spring 2011 schedule

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 26: Line 26:
| Thur Feb 3
| Thur Feb 3
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|
+
|[[User:Carrie M Thompson| CT]]
| [[20.109(S11):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
| [[20.109(S11):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
|
|
Line 34: Line 34:
| Tue Feb 8
| Tue Feb 8
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
| [[20.109(S11):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
|
|
Line 42: Line 42:
| Thur Feb 10
| Thur Feb 10
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
| [[20.109(S11):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
|
|
Line 50: Line 50:
| Tue Feb 15
| Tue Feb 15
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
| [[20.109(S11):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
|
|
Line 58: Line 58:
| Thur Feb 17
| Thur Feb 17
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
| [[20.109(S11):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
|
|
Line 71: Line 71:
| Thur Feb 24
| Thur Feb 24
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|
+
|[[User:Carrie M Thompson| CT]]
| [[20.109(S11):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
| [[20.109(S11):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
|
|
Line 79: Line 79:
| Tue Mar 1
| Tue Mar 1
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):Aptamer binding assay (Day7)| Aptamer binding assay]]
| [[20.109(S11):Aptamer binding assay (Day7)| Aptamer binding assay]]
|
|
Line 87: Line 87:
| Thur Mar 3
| Thur Mar 3
| [http://web.mit.edu/be/people/niles.shtml JN]
| [http://web.mit.edu/be/people/niles.shtml JN]
-
|  
+
| [[User:Carrie M Thompson| CT]]
| [[20.109(S11):Journal club (Day8)| Journal club]]  
| [[20.109(S11):Journal club (Day8)| Journal club]]  
|
|
Line 97: Line 97:
| Tue Mar 8
| Tue Mar 8
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall|  AR]]
|[[20.109(S11):Explore model system (Day1)| Explore model system]]
|[[20.109(S11):Explore model system (Day1)| Explore model system]]
|<font color = CC0000><b>Module 1 Draft Due</b></font color><br>
|<font color = CC0000><b>Module 1 Draft Due</b></font color><br>
Line 105: Line 105:
| Thur Mar 10
| Thur Mar 10
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall|  AR]]
|[[20.109(S11):Complete DNA design (Day2)| Complete DNA design]]
|[[20.109(S11):Complete DNA design (Day2)| Complete DNA design]]
|
|
Line 113: Line 113:
| Tue Mar 15
| Tue Mar 15
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall| AR]]
|[[20.109(S11):Prepare DNA for cloning (Day3)| Prepare DNA for cloning]]
|[[20.109(S11):Prepare DNA for cloning (Day3)| Prepare DNA for cloning]]
|
|
Line 121: Line 121:
| Thur Mar 17
| Thur Mar 17
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall|  AR]]
|[[20.109(S11):Ligate DNA and transform bacteria (Day4)| Ligate DNA and transform bacteria]]
|[[20.109(S11):Ligate DNA and transform bacteria (Day4)| Ligate DNA and transform bacteria]]
|
|
Line 134: Line 134:
| Tue Mar 29
| Tue Mar 29
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall|  AR]]
|[[20.109(S11):Prepare candidate clones in model cell strain (Day5)| Prepare candidate clones in model cell strain]]
|[[20.109(S11):Prepare candidate clones in model cell strain (Day5)| Prepare candidate clones in model cell strain]]
|
|
Line 142: Line 142:
| Thur Mar 31
| Thur Mar 31
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall| AR]]
|[[20.109(S11):Evaluate DNA and choose clone (Day6)| Evaluate DNA and choose clone]]
|[[20.109(S11):Evaluate DNA and choose clone (Day6)| Evaluate DNA and choose clone]]
|<font color = CC0000><b>Module 1 Final Report Due</b></font color><br>
|<font color = CC0000><b>Module 1 Final Report Due</b></font color><br>
Line 150: Line 150:
| Tue Apr 5
| Tue Apr 5
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall|  AR]]
|[[20.109(S11):Assay modified model system (Day7)| Assay modified model system]]
|[[20.109(S11):Assay modified model system (Day7)| Assay modified model system]]
|
|
Line 158: Line 158:
| Thur Apr 7
| Thur Apr 7
| [http://web.mit.edu/be/people/weiss.shtml RW]
| [http://web.mit.edu/be/people/weiss.shtml RW]
-
|  
+
| [[User:Adrian_Randall| AR]]
|[[20.109(S11):Wrap-up analysis and modelingb (Day8)| Wrap-up analysis and modeling]]
|[[20.109(S11):Wrap-up analysis and modelingb (Day8)| Wrap-up analysis and modeling]]
|
|
Line 168: Line 168:
| Tues Apr 12
| Tues Apr 12
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]]
| [[20.109(S11):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]]
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>
Line 176: Line 176:
| Thur Apr 14
| Thur Apr 14
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung|HHH]]
| [[20.109(S11):Initiate cell culture (Day2) | Initiate cell culture]]
| [[20.109(S11):Initiate cell culture (Day2) | Initiate cell culture]]
|
|
Line 189: Line 189:
| Thur Apr 21
| Thur Apr 21
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Testing cell viability (Day3) | Testing cell viability]]
| [[20.109(S11):Testing cell viability (Day3) | Testing cell viability]]
|
|
Line 197: Line 197:
| Tue Apr 26
| Tue Apr 26
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Preparing cells for analysis (Day4) | Preparing cells for analysis]]
| [[20.109(S11):Preparing cells for analysis (Day4) | Preparing cells for analysis]]
|
|
Line 205: Line 205:
| Thur Apr 28
| Thur Apr 28
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]]
| [[20.109(S11):Initiating transcript and protein assays (Day5)| Initiating transcript and protein assays]]
|<font color = CC0000><b>Module 2 Final Report Due</b></font color><br>
|<font color = CC0000><b>Module 2 Final Report Due</b></font color><br>
Line 213: Line 213:
| Tue May 3
| Tue May 3
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|
+
|[[User:Han-Hwa Hung| HHH]]]
| [[20.109(S11):Transcript-level analysis (Day6)| Transcript-level analysis]]
| [[20.109(S11):Transcript-level analysis (Day6)| Transcript-level analysis]]
|
|
Line 221: Line 221:
| Thur May 5
| Thur May 5
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
| [[20.109(S11):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
|  
|  
Line 229: Line 229:
| Tue May 10
| Tue May 10
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
-
|  
+
| [[User:Han-Hwa Hung| HHH]]
| [[20.109(S11):Student presentations (Day8) | Student presentations]]
| [[20.109(S11):Student presentations (Day8) | Student presentations]]
|
|

Revision as of 21:49, 30 January 2011

20.109(S11): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2011        Assignments        Lab Basics        OWW Basics       
RNA Engineering        System Engineering        Cell-Biomaterial Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 1 ANS Orientation
1 1 Thur Feb 3 JN CT Amplify aptamer-encoding DNA
1 2 Tue Feb 8 JN CT Purify aptamer-encoding DNA
1 3 Thur Feb 10 JN CT Prepare RNA by IVT
1 4 Tue Feb 15 JN CT Purify RNA and run affinity column
1 5 Thur Feb 17 JN CT RNA to DNA by RT-PCR
President's Day Feb 21 No lab Feb 22 or Feb 23
1 6 Thur Feb 24 JN CT Post-selection IVT and journal club
1 7 Tue Mar 1 JN CT Aptamer binding assay
1 8 Thur Mar 3 JN CT Journal club
2 1 Tue Mar 8 RW AR Explore model system Module 1 Draft Due
2 2 Thur Mar 10 RW AR Complete DNA design
2 3 Tue Mar 15 RW AR Prepare DNA for cloning
2 4 Thur Mar 17 RW AR Ligate DNA and transform bacteria
Spring Break Mar 21-25 No lab all week
2 5 Tue Mar 29 RW AR Prepare candidate clones in model cell strain
2 6 Thur Mar 31 RW AR Evaluate DNA and choose clone Module 1 Final Report Due
2 7 Tue Apr 5 RW AR Assay modified model system
2 8 Thur Apr 7 RW AR Wrap-up analysis and modeling
3 1 Tues Apr 12 ANS HHH Start-up biomaterials engineering Module 2 Draft Due
3 2 Thur Apr 14 ANS HHH Initiate cell culture
Patriot's Day April 18-19 No lab Apr 19 or Apr 20
3 3 Thur Apr 21 ANS HHH Testing cell viability
3 4 Tue Apr 26 ANS HHH Preparing cells for analysis
3 5 Thur Apr 28 ANS HHH Initiating transcript and protein assays Module 2 Final Report Due
3 6 Tue May 3 ANS HHH] Transcript-level analysis
3 7 Thur May 5 ANS HHH Protein-level and wrap-up analysis
3 8 Tue May 10 ANS HHH Student presentations
Thur May 12 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
Personal tools