20.109(S13): Spring 2013 schedule

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20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              


MODULE DAY DATE LECTURER TA LAB NOTES
Tue/Wed Feb 5/6 ANS Orientation * Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
* Intro Pre-Lab, W/F
1 1 Thur/Fri Feb 7/8 JR Ian Context-setting and primer design
1 2 Tue/Wed Feb 12/13 JR Ian DNA extraction
1 3 Thur/Fri Feb 14/15 JR Ian PCR and paper discussion
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur/Fri Feb 21/22 JR Ian DNA cloning
1 5 Tue/Wed Feb 26/27 JR Ian DNA sequencing and primer analysis
1 6 Thur/Fri Feb 28/ Mar 1 JR Ian Journal club I
1 7 Tue/Wed Mar 5/6 JR Ian Phylogenetic analysis
1 8 Thur/Fri Mar 7/8 NO LECTURE TODAY Journal club II
2 1 Tue/Wed Mar 12/13 AJ MM Design mutant
Module 1 Draft Due
2 2 Thur/Fri Mar 14/15 AJ MM Site-directed mutagenesis
2 3 Tue/Wed Mar 19/20 AJ MM Bacterial amplification of DNA
Module 1 Primer Summary Due
2 4 Thur/Fri Mar 21/22 AJ MM Prepare expression system
Spring Break Mar 25-29 No lab all week
2 5 Tue/Wed Apr 2/3 AJ MM Induce protein and evaluate DNA
2 6 Thur/Fri Apr 4/5 AJ MM Purify protein
Module 1 Revision Due
2 7 Tue/Wed Apr 9/10 AJ MM Characterize protein expression
2 8 Thur/Fri Apr 11/12 AJ MM Assess protein function
Patriot's Day April 15-16 No lab Apr 16 or Apr 17
3 1 Thur/Fri Apr 18/19 ANS Design experiment
3 2 Tue/Wed Apr 23/24 ANS Initiate cell culture
Module 2 Report Due MONDAY APRIL 22 by 5 pm for all.
3 3 Thur/Fri Apr 25/26 ANS Testing cell viability
3 4 Tue/Wed Apr 30/May 1 ANS Preparing cells for analysis
3 5 Thur/Fri May 2/3 ANS Initiating transcript and protein assays
3 6 Tue/Wed May 7/8 ANS Transcript-level analysis
3 7 Thur/Fri May 9/10 ANS Protein-level and wrap-up analysis
Module 3 Summary Due
3 8 Tue/Wed May 14/15 ANS Student presentations
Thur May 16 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
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