20.109(S13): TA notes for module 1

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(Day 2)
(Day 2)
Line 29: Line 29:
'''Materials required:'''  
'''Materials required:'''  
*Bird samples! At 100 μL each in a '''2 mL''' tube
*Bird samples! At 100 μL each in a '''2 mL''' tube
-
**Use filter pipet tip to transfer -- consult Agi about which samples to prepare
+
**Use filter pipet tip to transfer and store at -80 °C -- consult Agi about which samples to prepare
*Aliquots of components from QiaAMP stool kit (see GoogleDoc)
*Aliquots of components from QiaAMP stool kit (see GoogleDoc)
**Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
**Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
Line 37: Line 37:
'''Day of Lab (R/F):'''
'''Day of Lab (R/F):'''
 +
*Thaw that day's 100 μL samples in the fridge starting just before lecture. -Agi will do
 +
*Place 4 ice buckets around the room (between pairs) for keeping samples chilled at first.
 +
*Before lab, move samples to the ice bucket nearest the pair that will be using those particular samples (see table on Talk page, ready shortly).
*Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
*Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
*Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
*Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
-
*Either set up second heat block at 56 °C or turn on the small oven (may be easiest)
+
*Either set up second heat block at 56 °C or turn on the small oven (may be easiest). -Agi will do
'''After Lab:'''
'''After Lab:'''

Revision as of 10:11, 12 February 2013

20.109(S13): Laboratory Fundamentals of Biological Engineering

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Contents

General notes

See also GoogleDoc for aliquoting amounts.

Scheme:

Key preparation:

Day-by-day

Day 1

Materials required:

Day of Lab (R/F):

After Lab:

How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.

Day 2

Materials required:

  • Bird samples! At 100 μL each in a 2 mL tube
    • Use filter pipet tip to transfer and store at -80 °C -- consult Agi about which samples to prepare
  • Aliquots of components from QiaAMP stool kit (see GoogleDoc)
    • Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
    • Some aliquots may be approximate (use your judgment). For example, it will be easier to pipet approximately 0.5 mL into each tube using a serological pipet than to pipet exactly 0.46 mL eight times per day, times several reagents!
    • ASL and AL must be aliquoted the day of lab (see below)
  • InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one

Day of Lab (R/F):

  • Thaw that day's 100 μL samples in the fridge starting just before lecture. -Agi will do
  • Place 4 ice buckets around the room (between pairs) for keeping samples chilled at first.
  • Before lab, move samples to the ice bucket nearest the pair that will be using those particular samples (see table on Talk page, ready shortly).
  • Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
  • Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
  • Either set up second heat block at 56 °C or turn on the small oven (may be easiest). -Agi will do

After Lab:

  • Store extraction samples at -20 °C until next time
How it went:
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