20.109:Module 3

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 9: Line 9:
'''TA:''' [[Andrea Tentner]]
'''TA:''' [[Andrea Tentner]]
-
 
+
In this experimental module you will...
[[Image:Macintosh HD-Users-nkuldell-Desktop-ExpressionEng coverart S07.jpg|thumb|500 px|center| SAGA image from F.Winston<br> Mode of action model from P. Schultz <br> Mol Cell. 2004 Jul 23;15(2):199[[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15260971&query_hl=4&itool=pubmed_docsum]]<br> Microarray image from N. Kuldell<br>]]
[[Image:Macintosh HD-Users-nkuldell-Desktop-ExpressionEng coverart S07.jpg|thumb|500 px|center| SAGA image from F.Winston<br> Mode of action model from P. Schultz <br> Mol Cell. 2004 Jul 23;15(2):199[[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15260971&query_hl=4&itool=pubmed_docsum]]<br> Microarray image from N. Kuldell<br>]]
 +
 +
[[20.109(S07): Start-up expression engineering| Module 3 Day 1: Start-up expression engineering]]<br>
 +
[[20.109(S07): Yeast transformation| Module 3 Day 2: Yeast transformation]]<br>
 +
[[20.109(S07): Colony PCR| Module 3 Day 3: Colony PCR]] <br>
 +
[[20.109(S07): Screen for phenotypes, isolate RNA| Module 3 Day 4: Screen for phenotypes, isolate RNA]] <br>
 +
[[20.109(S07): cDNA synthesis and microarray| Module 3 Day 5: cDNA synthesis and microarray]] <br>
 +
[[20.109(S07): Microarray data analysis| Module 3 Day 6: Microarray data analysis]]<br>
 +
 +
 +
[[20.109(S07): TA's notes for module 3| TA notes, mod 3]]

Revision as of 11:51, 15 November 2006

20.109: Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Module 3

Instructors: Natalie Kuldell

TA: Andrea Tentner

In this experimental module you will...

SAGA image from F.Winston Mode of action model from P. Schultz  Mol Cell. 2004 Jul 23;15(2):199[[1]] Microarray image from N. Kuldell
SAGA image from F.Winston
Mode of action model from P. Schultz
Mol Cell. 2004 Jul 23;15(2):199[[1]]
Microarray image from N. Kuldell

Module 3 Day 1: Start-up expression engineering
Module 3 Day 2: Yeast transformation
Module 3 Day 3: Colony PCR
Module 3 Day 4: Screen for phenotypes, isolate RNA
Module 3 Day 5: cDNA synthesis and microarray
Module 3 Day 6: Microarray data analysis


TA notes, mod 3
Personal tools