20.109:Module 3

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20.109: Laboratory Fundamentals of Biological Engineering

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Module 3

Instructors: Natalie Kuldell

TA: Andrea Tentner

In this experimental module you will...

SAGA image from F.Winston
Mode of action model from P. Schultz
Mol Cell. 2004 Jul 23;15(2):199[[1]]
Microarray image from N. Kuldell

Module 3 Day 1: Start-up expression engineering
Module 3 Day 2: Yeast transformation
Module 3 Day 3: Colony PCR
Module 3 Day 4: Screen for phenotypes, isolate RNA
Module 3 Day 5: cDNA synthesis and microarray
Module 3 Day 6: Microarray data analysis


TA notes, mod 3

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