20.109:Module 4

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[[BE.109:Bio-material engineering/Screening library | Module 4 Day 1: Screening library]]<br>
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[[20.109(S07):Bio-material engineering/Screening library | Module 4 Day 1: Screening library]]<br>
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[[BE.109:Bio-material engineering/Optimizing panning | Day 2: Optimizing panning]]<br>
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[[20.109(S07): Bio-material engineering/Optimizing panning | Module 4 Day 2: Optimizing panning]]<br>
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[[BE.109:Bio-material engineering/Rescreening gold binders | Day 3: Rescreening gold binders]]<br>
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[[20.109(S07): Bio-material engineering/Rescreening gold binders | Module 4 Day 3: Rescreening gold binders]]<br>
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[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 4: PCR of gold binding candidates]]<br>
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[[20.109(S07): Bio-material engineering/PCR of gold binding candidates | Module 4 Day 4: PCR of gold binding candidates]]<br>
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[[BE.109:Presenting your work | Day 5: Student presentations]]<br>
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[[20.109 (S07): Research proposal, module 4| Module 4 Day 5: Student presentations]]<br>
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[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]<br>
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[[20.109(S07): Bio-material engineering/Sequence analysis | Module 4 Day 6: Sequence analysis]]<br>
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[[20.109(S07): Biomaterial engineering, TA notes| TA notes Module 4]]

Current revision

20.109: Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Module 4

Instructors: Angela Belcher and Natalie Kuldell

TA: Chandni Valiathan

In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we’ll elucidate some amino acid requirements for the yeast/metal interaction.

S. cerevisiae shown budding (top) and bound to CdS (bottom)


Module 4 Day 1: Screening library
Module 4 Day 2: Optimizing panning
Module 4 Day 3: Rescreening gold binders
Module 4 Day 4: PCR of gold binding candidates
Module 4 Day 5: Student presentations
Module 4 Day 6: Sequence analysis

TA notes Module 4
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