20.109: 2-component signaling: Difference between revisions

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*Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli. Siryaporn A, Goulian M. Mol Microbiol. 2008 Oct;70(2):494-506. Epub 2008 Aug 29. [[PMID: 18761686]] and [[Media:Crosstalk MolMicro08.pdf| pdf]]  
*Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli. Siryaporn A, Goulian M. Mol Microbiol. 2008 Oct;70(2):494-506. Epub 2008 Aug 29. [[PMID: 18761686]] and [[Media:Crosstalk MolMicro08.pdf| pdf]]  
*Design and signaling mechanism of light-regulated histidine kinases. Möglich A, Ayers RA, Moffat K. J Mol Biol. 2009 Feb 6;385(5):1433-44. Epub 2008 Dec 14. [[PMID: 19109976]] and [[Media:Light-reg2CS JMB09.pdf|pdf]]
*Design and signaling mechanism of light-regulated histidine kinases. Möglich A, Ayers RA, Moffat K. J Mol Biol. 2009 Feb 6;385(5):1433-44. Epub 2008 Dec 14. [[PMID: 19109976]] and [[Media:Light-reg2CS JMB09.pdf|pdf]]
*EnvZ-OmpR interaction and osmoregulation in Escherichia coli. Cai SJ, Inouye M. J Biol Chem. 2002 Jul 5;277(27):24155-61. Epub 2002 Apr 24. [[PMID: 11973328]] and [[Media:OsmoregEnvZ-OmpR JBC02.pdf| pdf]]
==K-P+ and K+P- mutations==
==K-P+ and K+P- mutations==
06.16.09 email from Mike:  
06.16.09 email from Mike:  

Revision as of 10:04, 22 June 2009

Useful links

Useful Images and Files

Coliroid Signaling


Coliroid example


plasmid map of pCph8, file is here


pCph8 cut sites, zero-cutters in red

References

  • Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA.

Nature. 2005 Nov 24;438(7067):441-2. PMID: 16306980 and pdf

  • Engineered single- and multi-cell chemotaxis pathways in E. coli. Goldberg SD, Derr P, DeGrado WF, Goulian M. Mol Syst Biol. 2009;5:283. Epub 2009 Jun 16. PMID: 19536206 and pdf
  • Specificity in two-component signal transduction pathways. Laub MT, Goulian M. Annu Rev Genet. 2007;41:121-45. Review. PMID: 18076326 and pdf
  • Mutations that alter the kinase and phosphatase activities of the two-component sensor EnvZ. Hsing W, Russo FD, Bernd KK, Silhavy TJ. J Bacteriol. 1998 Sep;180(17):4538-46. PMID: 9721293 and pdf
  • Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli. Siryaporn A, Goulian M. Mol Microbiol. 2008 Oct;70(2):494-506. Epub 2008 Aug 29. PMID: 18761686 and pdf
  • Design and signaling mechanism of light-regulated histidine kinases. Möglich A, Ayers RA, Moffat K. J Mol Biol. 2009 Feb 6;385(5):1433-44. Epub 2008 Dec 14. PMID: 19109976 and pdf
  • EnvZ-OmpR interaction and osmoregulation in Escherichia coli. Cai SJ, Inouye M. J Biol Chem. 2002 Jul 5;277(27):24155-61. Epub 2002 Apr 24. PMID: 11973328 and pdf

K-P+ and K+P- mutations

06.16.09 email from Mike: As for the EnvZ mutants, here's the list we've compiled from the literature. Keep in mind that K+P- really means a shift in the balance of kinase and phosphatase activities and similarly for the K-P+ alleles. None of them is perfectly "clean" in eliminating one of the activities:

K+P-

  • V241G
  • G240E
  • S242D
  • P248Q
  • T247R
  • Q283P
  • Y287D
  • L288P

K-P+

  • A239T
  • N343K
  • F390L
  • H243A

NK note: relevant reference:

  • Mutations that alter the kinase and phosphatase activities of the two-component sensor EnvZ. Hsing W, Russo FD, Bernd KK, Silhavy TJ. J Bacteriol. 1998 Sep;180(17):4538-46. PMID: 9721293
  • The role of the G2 box, a conserved motif in the histidine kinase superfamily, in modulating the function of EnvZ. Zhu Y, Inouye M. Mol Microbiol. 2002 Aug;45(3):653-63. PMID: 12139613
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