20.109: Spring 2007 schedule

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|'''TA'''
|'''TA'''
|'''LAB'''
|'''LAB'''
 +
|'''note'''
|--
|--
|  
|  
|  
|  
-
| Tues Feb 6  
+
| Tue Feb 6  
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
|  
|  
-
| [[BE.109:Orientation | Orientation]]
+
| [[20.109(S07):Lab tour | Orientation]]
 +
|
|--
|--
-
| colspan="6" |
+
| colspan="7" |
|--
|--
| 1
| 1
| 1
| 1
-
| Thurs Feb 8
+
| Thur Feb 8
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
| [[20.109(S07):Start-up genome engineering | Start-up genome engineering]]
| [[20.109(S07):Start-up genome engineering | Start-up genome engineering]]
 +
| [[Media:20109080207.pdf | PDF]] of class discussion slides
|--
|--
| 1
| 1
| 2
| 2
-
| Tues Feb 13  
+
| Tue Feb 13  
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
| [[20.109(S07): Agarose gel electrophoresis| Agarose gel electrophoresis]]
| [[20.109(S07): Agarose gel electrophoresis| Agarose gel electrophoresis]]
 +
| [[Media:20109M1L2.pdf | PDF]] of class notes
|--
|--
| 1
| 1
| 3
| 3
-
| Thurs Feb 15
+
| Thur Feb 15
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
| [[20.109(S07): DNA ligation and bacterial transformation| DNA ligation and bacterial transformation]]
| [[20.109(S07): DNA ligation and bacterial transformation| DNA ligation and bacterial transformation]]
 +
|
|--
|--
|
|
|
|
-
! colspan="3" | President's Day Feb 19
+
! colspan="4" | President's Day Feb 19
-
| '''No lab Feb 20 (Mon schedule) or Feb 21'''
+
| '''No lab Feb 20 or Feb 21'''
|--
|--
| 1
| 1
| 4
| 4
-
| Thurs Feb 22
+
| Thur Feb 22
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
-
|  
+
| [[20.109(S07): Examine candidate clones| Examine candidate clones]]
 +
| guest lecture from Harlan Breindel
|--
|--
| 1
| 1
| 5
| 5
-
| Tues Feb 27
+
| Tue Feb 27
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
-
|  
+
| [[20.109(S07): Western analysis| Western analysis]]
 +
| [[Media:2010927022007.pdf | PDF]] of class discussion slides
|--
|--
| 1
| 1
| 6
| 6
-
| Thurs Mar 1
+
| Thur Mar 1
| [[Drew Endy | DE]]
| [[Drew Endy | DE]]
| [[Sean Clarke | SC]]
| [[Sean Clarke | SC]]
-
|  
+
| [[20.109(S07): Probe western| Probe western]]
 +
| first draft of [[20.109(S07): Genome engineering essay| your writing assignment]] due before lab, [[Media:2010901032007.pdf | PDF]] of class slides
|--
|--
-
| colspan="6" |
+
| colspan="7" |
|--
|--
| 2
| 2
| 1
| 1
-
| Tues Mar 6  
+
| Tue Mar 6  
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
| [[BE.109:Protein engineering/Tools for protein engineering | Tools for protein engineering]]
+
| [[20.109(S07): Start-up signal measurement| Start-up signal measurement]]
 +
|
|--
|--
| 2
| 2
| 2
| 2
-
| Thurs Mar 8
+
| Thur Mar 8
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
|  
+
| [[20.109(S07): Measuring calcium in vitro| Measuring calcium in vitro]]
 +
|
|--
|--
| 2
| 2
| 3
| 3
-
| Tues Mar 13
+
| Tue Mar 13
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
|  
+
| [[20.109(S07): Lipofection| Lipofection]]
 +
|
|--
|--
| 2
| 2
| 4
| 4
-
| Thurs Mar 15
+
| Thur Mar 15
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
|  
+
| [[20.109(S07): Measuring calcium in vivo| Measuring calcium in vivo]]
 +
|
|--
|--
| 2
| 2
| 5
| 5
-
| Tues Mar 20  
+
| Tue Mar 20  
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
|  
+
| [[20.109(S07): Calcium signaling in vivo| Calcium signaling in vivo]]
 +
|
|--
|--
| 2
| 2
| 6
| 6
-
| Thurs Mar 22
+
| Thur Mar 22
| AJ
| AJ
| [[Yoon Sung Nam | YSN]]
| [[Yoon Sung Nam | YSN]]
-
| [[BE.109:Presenting your work | Student presentations]]
+
| [[20.109(S07): Student presentations, module 2 | Student presentations]]
 +
|
|--
|--
|
|
|
|
-
! colspan="3" | Spring Break Mar 26-30
+
! colspan="4" | Spring Break Mar 26-30
| '''No lab all week'''
| '''No lab all week'''
|--
|--
-
| colspan="6" |
+
| colspan="7" |
|--
|--
| 3
| 3
| 1
| 1
-
| Tues Apr 3
+
| Tue Apr 3
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
| [[BE.109:Systems engineering/Tools for systems engineering | Tools for systems engineering]]
+
| [[20.109(S07): Start-up expression engineering| Start-up expression engineering]]
 +
| [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect1 handout.pdf| PDF]] of M3D1 lecture slides 
|--
|--
| 3
| 3
| 2
| 2
-
| Thurs Apr 5
+
| Thur Apr 5
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
|  
+
| [[20.109(S07): Yeast transformation| Yeast transformation]]
 +
| [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect2 handout.pdf| PDF]] of M3D2 lecture slides
|--
|--
| 3
| 3
| 3
| 3
-
| Tues Apr 10  
+
| Tue Apr 10  
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
|  
+
| [[20.109(S07): Colony PCR| Colony PCR]]
 +
| guest lecture: Susan Ruff and her slides are [[Media:Macintosh HD-Users-nkuldell-Desktop-What is I M R D Anyway.pdf| here]]
|--
|--
| 3
| 3
| 4
| 4
-
| Thurs Apr 12  
+
| Thur Apr 12  
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
|  
+
| [[20.109(S07): Screen for phenotypes, isolate RNA| Screen for phenotypes, isolate RNA]]
 +
| [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect3 handout.pdf | PDF]] of M3D4 lecture slides
|--
|--
|
|
|
|
-
! colspan="3" | Patriot's Day April 16-17
+
! colspan="4" | Patriot's Day April 16-17
| '''No lab Apr 17 or Apr 18'''
| '''No lab Apr 17 or Apr 18'''
|--
|--
| 3
| 3
| 5
| 5
-
| Thurs Apr 19
+
| Thur Apr 19
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
|  
+
| [[20.109(S07): cDNA synthesis and microarray| cDNA synthesis and microarray]]
 +
| [[Media:109(S07)lect4 handout.pdf| PDF]] of M3D5 lecture slides
|--
|--
| 3
| 3
| 6
| 6
-
| Tues Apr 24
+
| Tue Apr 24
| [[Natalie Kuldell | NK]]
| [[Natalie Kuldell | NK]]
| [[Andrea Tentner | AT]]
| [[Andrea Tentner | AT]]
-
|  
+
| [[20.109(S07): Microarray data analysis| Microarray data analysis]]
 +
| guest lecture: Rebecca Fry <br> and her slides are [[Media:Macintosh HD-Users-nkuldell-Desktop-dataanalysis20071.pdf| here]]
|--
|--
-
| colspan="6" |
+
| colspan="7" |
|--
|--
| 4
| 4
| 1
| 1
-
| Thurs Apr 26
+
| Thur Apr 26
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
 +
| [[20.109(S07):Bio-material engineering/Screening library | Screening library]]
|  
|  
|--
|--
| 4
| 4
| 2
| 2
-
| Tues May 1  
+
| Tue May 1  
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
-
|  
+
| [[20.109(S07): Bio-material engineering/Optimizing panning | Optimizing panning]]
 +
| first draft of [[20.109(S07): Expression engineering report| your writing assignment]] due Monday April 30th by 9AM
|--
|--
| 4
| 4
| 3
| 3
-
| Thurs May 3
+
| Thur May 3
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
 +
| [[20.109(S07): Bio-material engineering/Rescreening gold binders | Rescreening gold binders]]
|  
|  
|--
|--
| 4
| 4
| 4
| 4
-
| Tues May 8
+
| Tue May 8
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
-
|  
+
| [[20.109(S07): Bio-material engineering/PCR of gold binding candidates | PCR of gold binding candidates]]
 +
| Mod 3 lab reports returned for revision
|--
|--
| 4
| 4
| 5
| 5
-
| Thurs May 10
+
| Thur May 10
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
 +
| [[20.109 (S07): Research proposal, module 4| Student presentations]]
|  
|  
|--
|--
| 4
| 4
| 6
| 6
-
| Tues May 15  
+
| Tue May 15  
| AB
| AB
| [[Chandni Valiathan | CV]]
| [[Chandni Valiathan | CV]]
-
| [[BE.109:Presenting your work | Student presentations]]
+
| [[20.109(S07): Bio-material engineering/Sequence analysis | Analyze sequence data]]
 +
| Revised Mod 3 writing assignment due before lab<br> no lecture today
|--
|--
|
|
|
|
-
| Thurs May 18
+
| Thur May 17
|
|
|
|
| Lecture only, no lab
| Lecture only, no lab
 +
|
|}
|}
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Current revision

20.109: Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 6 NK Orientation
1 1 Thur Feb 8 DE SC Start-up genome engineering PDF of class discussion slides
1 2 Tue Feb 13 DE SC Agarose gel electrophoresis PDF of class notes
1 3 Thur Feb 15 DE SC DNA ligation and bacterial transformation
President's Day Feb 19 No lab Feb 20 or Feb 21
1 4 Thur Feb 22 DE SC Examine candidate clones guest lecture from Harlan Breindel
1 5 Tue Feb 27 DE SC Western analysis PDF of class discussion slides
1 6 Thur Mar 1 DE SC Probe western first draft of your writing assignment due before lab, PDF of class slides
2 1 Tue Mar 6 AJ YSN Start-up signal measurement
2 2 Thur Mar 8 AJ YSN Measuring calcium in vitro
2 3 Tue Mar 13 AJ YSN Lipofection
2 4 Thur Mar 15 AJ YSN Measuring calcium in vivo
2 5 Tue Mar 20 AJ YSN Calcium signaling in vivo
2 6 Thur Mar 22 AJ YSN Student presentations
Spring Break Mar 26-30 No lab all week
3 1 Tue Apr 3 NK AT Start-up expression engineering PDF of M3D1 lecture slides
3 2 Thur Apr 5 NK AT Yeast transformation PDF of M3D2 lecture slides
3 3 Tue Apr 10 NK AT Colony PCR guest lecture: Susan Ruff and her slides are here
3 4 Thur Apr 12 NK AT Screen for phenotypes, isolate RNA PDF of M3D4 lecture slides
Patriot's Day April 16-17 No lab Apr 17 or Apr 18
3 5 Thur Apr 19 NK AT cDNA synthesis and microarray PDF of M3D5 lecture slides
3 6 Tue Apr 24 NK AT Microarray data analysis guest lecture: Rebecca Fry
and her slides are here
4 1 Thur Apr 26 AB CV Screening library
4 2 Tue May 1 AB CV Optimizing panning first draft of your writing assignment due Monday April 30th by 9AM
4 3 Thur May 3 AB CV Rescreening gold binders
4 4 Tue May 8 AB CV PCR of gold binding candidates Mod 3 lab reports returned for revision
4 5 Thur May 10 AB CV Student presentations
4 6 Tue May 15 AB CV Analyze sequence data Revised Mod 3 writing assignment due before lab
no lecture today
Thur May 17 Lecture only, no lab
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