| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
|
|
|
Tues Feb
|
NK
|
|
Orientation
|
|
|
| 1
|
1
|
Thurs Feb
|
|
|
DNA engineering by PCR
|
| 1
|
2
|
Tues Feb
|
|
|
Clean and cut DNA
|
| 1
|
3
|
Thurs
|
|
|
Agarose gel electrophoresis
|
|
|
|
President's Day Feb 20
|
No lab Feb 21 (Mon schedule) or Feb 22
|
| 1
|
4
|
Thurs
|
|
|
DNA ligation and bacterial transformation
|
| 1
|
5
|
Tues Feb
|
|
|
Examine candidate clones
|
| 1
|
6
|
Thurs
|
|
|
Restriction map+ Article Discussion/Tissue Culture
|
| 1
|
7
|
Tues
|
|
|
Lipofection
|
| 1
|
8
|
Thurs Mar
|
|
|
FACS analysis
|
|
|
| 2
|
1
|
Tues Mar
|
|
|
Tools for protein engineering
|
| 2
|
2
|
Thurs Mar
|
|
|
Assessing beta-galactosidase
|
| 2
|
3
|
Tues Mar
|
|
|
Purifying beta-galactosidase
|
| 2
|
4
|
Thurs Mar
|
|
|
Student presentations
|
|
|
|
Spring Break Mar 27-31
|
No lab Mar 27-31
|
|
|
| 3
|
1
|
Tues Apr
|
|
|
Tools for systems engineering
|
| 3
|
2
|
Thurs Apr
|
|
|
Basic bacterial photography: black and white
|
| 3
|
3
|
Tues Apr
|
|
|
Advanced bacterial photography: 2 color
|
| 3
|
4
|
Thurs Apr
|
|
|
Device characterization
|
|
|
|
Patriot's Day April 17-18
|
No lab Apr 18 or Apr 19
|
| 3
|
5
|
Thurs Apr
|
|
|
Measuring DNA, RNA, protein
|
| 3
|
6
|
Tues Apr
|
|
|
RT-PCR data analysis
|
|
|
| 4
|
1
|
Thurs Apr
|
|
|
Screening library
|
| 4
|
2
|
Tues May
|
|
|
Optimizing panning
|
| 4
|
3
|
Thurs May
|
|
|
Rescreening gold binders
|
| 4
|
4
|
Tues May
|
|
|
PCR of gold binding candidates
|
| 4
|
5
|
Thurs May
|
|
|
Student presentations
|
| 4
|
6
|
Tues May
|
|
|
Analyze sequence data
|
|
|
|
Thurs May 18
|
|
|
Lecture only, no lab
|
