20.109: Spring 2007 schedule: Difference between revisions
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| [[Sean Clarke | SC]] | | [[Sean Clarke | SC]] | ||
| [[20.109(S07):Start-up genome engineering | Start-up genome engineering]] | | [[20.109(S07):Start-up genome engineering | Start-up genome engineering]] | ||
| | | [[Media:20109080207.pdf | PDF]] of class discussion slides | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 36: | Line 36: | ||
| [[Sean Clarke | SC]] | | [[Sean Clarke | SC]] | ||
| [[20.109(S07): Agarose gel electrophoresis| Agarose gel electrophoresis]] | | [[20.109(S07): Agarose gel electrophoresis| Agarose gel electrophoresis]] | ||
| | | [[Media:20109M1L2.pdf | PDF]] of class notes | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 57: | Line 57: | ||
| [[Sean Clarke | SC]] | | [[Sean Clarke | SC]] | ||
| [[20.109(S07): Examine candidate clones| Examine candidate clones]] | | [[20.109(S07): Examine candidate clones| Examine candidate clones]] | ||
| | | guest lecture from Harlan Breindel | ||
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| 1 | | 1 | ||
| Line 65: | Line 65: | ||
| [[Sean Clarke | SC]] | | [[Sean Clarke | SC]] | ||
| [[20.109(S07): Western analysis| Western analysis]] | | [[20.109(S07): Western analysis| Western analysis]] | ||
| | | [[Media:2010927022007.pdf | PDF]] of class discussion slides | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 73: | Line 73: | ||
| [[Sean Clarke | SC]] | | [[Sean Clarke | SC]] | ||
| [[20.109(S07): Probe western| Probe western]] | | [[20.109(S07): Probe western| Probe western]] | ||
| first draft of [[20.109(S07): Genome engineering essay| your writing assignment]] due before lab | | first draft of [[20.109(S07): Genome engineering essay| your writing assignment]] due before lab, [[Media:2010901032007.pdf | PDF]] of class slides | ||
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| colspan="7" | | | colspan="7" | | ||
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| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): Start-up expression engineering| Start-up expression engineering]] | | [[20.109(S07): Start-up expression engineering| Start-up expression engineering]] | ||
| | | [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect1 handout.pdf| PDF]] of M3D1 lecture slides | ||
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| 3 | | 3 | ||
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| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): Yeast transformation| Yeast transformation]] | | [[20.109(S07): Yeast transformation| Yeast transformation]] | ||
| | | [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect2 handout.pdf| PDF]] of M3D2 lecture slides | ||
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| 3 | | 3 | ||
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| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): Colony PCR| Colony PCR]] | | [[20.109(S07): Colony PCR| Colony PCR]] | ||
| guest lecture: | | guest lecture: Susan Ruff and her slides are [[Media:Macintosh HD-Users-nkuldell-Desktop-What is I M R D Anyway.pdf| here]] | ||
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| 3 | | 3 | ||
| Line 162: | Line 162: | ||
| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): Screen for phenotypes, isolate RNA| Screen for phenotypes, isolate RNA]] | | [[20.109(S07): Screen for phenotypes, isolate RNA| Screen for phenotypes, isolate RNA]] | ||
| | | [[Media:Macintosh HD-Users-nkuldell-Desktop-lectures-BE109 lectures (S07)-109(S07)lect3 handout.pdf | PDF]] of M3D4 lecture slides | ||
|-- | |-- | ||
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| Line 175: | Line 175: | ||
| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): cDNA synthesis and microarray| cDNA synthesis and microarray]] | | [[20.109(S07): cDNA synthesis and microarray| cDNA synthesis and microarray]] | ||
| | | [[Media:109(S07)lect4 handout.pdf| PDF]] of M3D5 lecture slides | ||
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| 3 | | 3 | ||
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| [[Andrea Tentner | AT]] | | [[Andrea Tentner | AT]] | ||
| [[20.109(S07): Microarray data analysis| Microarray data analysis]] | | [[20.109(S07): Microarray data analysis| Microarray data analysis]] | ||
| guest lecture: Rebecca Fry | | guest lecture: Rebecca Fry <br> and her slides are [[Media:Macintosh HD-Users-nkuldell-Desktop-dataanalysis20071.pdf| here]] | ||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| | | [[20.109(S07):Bio-material engineering/Screening library | Screening library]] | ||
| | |||
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| 4 | | 4 | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| [[20.109(S07): | | [[20.109(S07): Bio-material engineering/Optimizing panning | Optimizing panning]] | ||
| | | first draft of [[20.109(S07): Expression engineering report| your writing assignment]] due Monday April 30th by 9AM | ||
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| 4 | | 4 | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| [[20.109(S07): | | [[20.109(S07): Bio-material engineering/Rescreening gold binders | Rescreening gold binders]] | ||
| | | | ||
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| 4 | | 4 | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| | | [[20.109(S07): Bio-material engineering/PCR of gold binding candidates | PCR of gold binding candidates]] | ||
| | | Mod 3 lab reports returned for revision | ||
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| 4 | | 4 | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| | | [[20.109 (S07): Research proposal, module 4| Student presentations]] | ||
| | | | ||
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| 4 | | 4 | ||
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| AB | | AB | ||
| [[Chandni Valiathan | CV]] | | [[Chandni Valiathan | CV]] | ||
| [[20.109(S07): | | [[20.109(S07): Bio-material engineering/Sequence analysis | Analyze sequence data]] | ||
| | | Revised Mod 3 writing assignment due before lab<br> no lecture today | ||
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| | | | ||
| | | | ||
| Thur May | | Thur May 17 | ||
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Latest revision as of 14:00, 23 April 2007
| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 6 | NK | Orientation | ||||
| 1 | 1 | Thur Feb 8 | DE | SC | Start-up genome engineering | PDF of class discussion slides |
| 1 | 2 | Tue Feb 13 | DE | SC | Agarose gel electrophoresis | PDF of class notes |
| 1 | 3 | Thur Feb 15 | DE | SC | DNA ligation and bacterial transformation | |
| President's Day Feb 19 | No lab Feb 20 or Feb 21 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 22 | DE | SC | Examine candidate clones | guest lecture from Harlan Breindel |
| 1 | 5 | Tue Feb 27 | DE | SC | Western analysis | PDF of class discussion slides |
| 1 | 6 | Thur Mar 1 | DE | SC | Probe western | first draft of your writing assignment due before lab, PDF of class slides |
| 2 | 1 | Tue Mar 6 | AJ | YSN | Start-up signal measurement | |
| 2 | 2 | Thur Mar 8 | AJ | YSN | Measuring calcium in vitro | |
| 2 | 3 | Tue Mar 13 | AJ | YSN | Lipofection | |
| 2 | 4 | Thur Mar 15 | AJ | YSN | Measuring calcium in vivo | |
| 2 | 5 | Tue Mar 20 | AJ | YSN | Calcium signaling in vivo | |
| 2 | 6 | Thur Mar 22 | AJ | YSN | Student presentations | |
| Spring Break Mar 26-30 | No lab all week | |||||
| 3 | 1 | Tue Apr 3 | NK | AT | Start-up expression engineering | PDF of M3D1 lecture slides |
| 3 | 2 | Thur Apr 5 | NK | AT | Yeast transformation | PDF of M3D2 lecture slides |
| 3 | 3 | Tue Apr 10 | NK | AT | Colony PCR | guest lecture: Susan Ruff and her slides are here |
| 3 | 4 | Thur Apr 12 | NK | AT | Screen for phenotypes, isolate RNA | PDF of M3D4 lecture slides |
| Patriot's Day April 16-17 | No lab Apr 17 or Apr 18 | |||||
| 3 | 5 | Thur Apr 19 | NK | AT | cDNA synthesis and microarray | PDF of M3D5 lecture slides |
| 3 | 6 | Tue Apr 24 | NK | AT | Microarray data analysis | guest lecture: Rebecca Fry and her slides are here |
| 4 | 1 | Thur Apr 26 | AB | CV | Screening library | |
| 4 | 2 | Tue May 1 | AB | CV | Optimizing panning | first draft of your writing assignment due Monday April 30th by 9AM |
| 4 | 3 | Thur May 3 | AB | CV | Rescreening gold binders | |
| 4 | 4 | Tue May 8 | AB | CV | PCR of gold binding candidates | Mod 3 lab reports returned for revision |
| 4 | 5 | Thur May 10 | AB | CV | Student presentations | |
| 4 | 6 | Tue May 15 | AB | CV | Analyze sequence data | Revised Mod 3 writing assignment due before lab no lecture today |
| Thur May 17 | Lecture only, no lab | |||||
