20.109: Spring 2007 schedule

20.109: Laboratory Fundamentals of Biological Engineering

Home People Schedule Spring 2007 Lab Basics OWW Basics
Genome Engineering Biophysical Signal Measurement Expression Engineering Biomaterial Engineering

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 6	NK		Orientation

1	1	Thur Feb 8	DE	SC	Start-up genome engineering
1	2	Tue Feb 13	DE	SC	Agarose gel electrophoresis
1	3	Thur Feb 15	DE	SC	DNA ligation and bacterial transformation
		President's Day Feb 19				No lab Feb 20 or Feb 21
1	4	Thur Feb 22	DE	SC	Examine candidate clones
1	5	Tue Feb 27	DE	SC	Western analysis
1	6	Thur Mar 1	DE	SC	Probe western	first draft of your writing assignment due before lab

2	1	Tue Mar 6	AJ	YSN	Start-up signal measurement
2	2	Thur Mar 8	AJ	YSN	Measuring calcium in vitro
2	3	Tue Mar 13	AJ	YSN	Lipofection
2	4	Thur Mar 15	AJ	YSN	Measuring calcium in vivo
2	5	Tue Mar 20	AJ	YSN	Calcium signaling in vivo
2	6	Thur Mar 22	AJ	YSN	Student presentations
		Spring Break Mar 26-30				No lab all week

3	1	Tue Apr 3	NK	AT	Start-up expression engineering
3	2	Thur Apr 5	NK	AT	Yeast transformation
3	3	Tue Apr 10	NK	AT	Colony PCR	guest lecture: Harlan Breindel
3	4	Thur Apr 12	NK	AT	Screen for phenotypes, isolate RNA
		Patriot's Day April 16-17				No lab Apr 17 or Apr 18
3	5	Thur Apr 19	NK	AT	cDNA synthesis and microarray
3	6	Tue Apr 24	NK	AT	Microarray data analysis	guest lecture: Rebecca Fry

4	1	Thur Apr 26	AB	CV	Growth of phage materials	first draft of your writing assignment due before lab
4	2	Tue May 1	AB	CV	Testing redesigned genomes
4	3	Thur May 3	AB	CV	Building phage nanowires	Mod 3 writing assignment returned for revision
4	4	Tue May 8	AB	CV	TEM
4	5	Thur May 10	AB	CV	AFM	Revised Mod 3 writing assignment due before lab
4	6	Tue May 15	AB	CV	Student presentations
		Thur May 18			Lecture only, no lab

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