20.309:Presentations

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#[http://www.physics.berkeley.edu/research/liphardt/pdfs/probe.pdf Y. Nakayama, ''et al.'', "Tunable nanowire nonlinear optical probe."  ''Nature'' '''447''', pp. 1098-1101 (2007).]
#[http://www.physics.berkeley.edu/research/liphardt/pdfs/probe.pdf Y. Nakayama, ''et al.'', "Tunable nanowire nonlinear optical probe."  ''Nature'' '''447''', pp. 1098-1101 (2007).]
#[http://www.physics.berkeley.edu/research/liphardt/pdfs/EColi.pdf JM. Walter, ''et al.'', "Light-powering Escherichia coli with proteorhodopsin" ''Proceedings of the National Academy of Sciences'' '''104''', pp. 2408–2412 (2007).] Stephanie Nix, Kate B
#[http://www.physics.berkeley.edu/research/liphardt/pdfs/EColi.pdf JM. Walter, ''et al.'', "Light-powering Escherichia coli with proteorhodopsin" ''Proceedings of the National Academy of Sciences'' '''104''', pp. 2408–2412 (2007).] Stephanie Nix, Kate B
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#[http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/miller_science2002.pdf M. J. Miller ''et al.'', "Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node," ''Science'' '''296''' pp. 1869-73 (2002).]
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#[http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/miller_science2002.pdf M. J. Miller ''et al.'', "Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node," ''Science'' '''296''' pp. 1869-73 (2002).] Luis Somoza
#[http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/wang_bj2005.pdf H. Wang ''et al.'', "Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissues," ''Biophys. J'' '''89'''(1), pp. 581-91 (2005).]
#[http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/wang_bj2005.pdf H. Wang ''et al.'', "Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissues," ''Biophys. J'' '''89'''(1), pp. 581-91 (2005).]
# [http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/hanson_BJ2002.pdf K. M. Hanson ''et al.'', "Two-Photon Fluorescence Lifetime Imaging of the Skin Stratum Corneum pH Gradient" ''Biophys. J'' '''83'''(3) pp. 1682-90 (2002).]
# [http://www.media.mit.edu/nanoscale/courses/BE309/private/Presentations/Sessions345/hanson_BJ2002.pdf K. M. Hanson ''et al.'', "Two-Photon Fluorescence Lifetime Imaging of the Skin Stratum Corneum pH Gradient" ''Biophys. J'' '''83'''(3) pp. 1682-90 (2002).]

Revision as of 02:16, 23 September 2008

20.309: Biological Instrumentation and Measurement

Home        Course Information        Schedule        People        Student Presentations        LAB SIGNUP       

Contents

Oct 10: Nucleic acid technologies

  1. J. W. Hong, et al. "A nanoliter-scale nucleic acid processor with parallel architecture," Nature Biotech. 22(4): pp. 435-439 (2004). Tiffany Guo
  2. L Warren, et al. "Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR" Proc. Nat. Acad. Sci. 2006. OR E.A. Ottesen et al. "Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria" Science 2006.
  3. E. Winfree, et al. "Design and self-assembly of two-dimensional DNA crystals," Nature 394(6693): pp. 539-544 (1998). AND P. W. K. Rothemund "Folding DNA to create nanoscale shapes and patterns," Nature 440(7082): pp. 297-302(2006).; Jerzy Szablowski

Oct 14 and Oct 24: Optical Microscopy: Imaging

  1. Z. E. Perlman et al., "Multidimensional Drug Profiling by Automated Microscopy," Science 306 pp. 1194-98 (2004). Rebecca Adams (Oct. 14)
  2. D. Axelrod, "Total Internal Reflection Fluorescence Microscopy in Cell Biology," Traffic 2 pp. 764-774 (2001). Roshini Zachariah
  3. E. Chung, D. Kim, and P. T. C. So, "Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy," Opt. Lett. 31(7) pp. 945-7 (2006).
  4. T. Ichimura et al., "Application of tip-enhanced microscopy for nonlinear Raman spectroscopy," Appl. Phys. Lett. 84(10), pp. 1768-70 (2004).
  5. T-W. Koo, S. Chan, and A. A. Berlin, "Single-molecule detection of biomolecules by surface-enhanced coherent anti-Stokes Raman scattering," Opt. Lett. 30(9), pp. 1024-6 (2005).
  6. M. J. Rust, M. Bates, X. Zhuang, "Sub-diffraction-limit imaging by stochastic reconstruction optical microscopy (STORM)," Nature Methods 3:793-795 (2006).
  7. Design of Fluorescence Wide Field Microscopy (2 person)

Nov 14: Nucleic acid technologies II

  1. J. M. Nam, C. S. Thaxton, C. A. Mirkin "Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins," Science 301(5641): pp. 1884-1886 (2003). Qing Han, Becky Kusko
  2. 20.309 Lab Module 1 - measuring DNA melting curves
  3. A. Engell and D. J. Muller "Observing single biomolecules at work with the atomic force microscope," Nature Stuct. Biol. 7(9): pp. 715-718 (2000).
  4. D. Rugar et al. "Single spin detection by magnetic resonance force microscopy," Nature 430(6997): pp. 329-332 (2004).Kevin Vogelsang

Nov 20: Scanning probe microscopy

  1. G. E. Fantner et al. "Sacrificial bonds and hidden length: Unraveling molecular mesostructures in tough materials" Biophys. J 90(4): pp. 1411-1418 (2006).
  2. SY Lee et al. "Chemomechanical mapping of ligand-receptor binding kinetics on cells" PNAS 104: pp. 9609-9614 (2007).Aditya Kohli, Dawn Spelke
  3. MJ Rosenbluth, WA. Lam, and DA Fletcher, “Force Microscopy of Nonadherent Cells: A Comparison of Leukemia Cell Deformability” Biophysical Journal 90: pp. 2994-3003 (2006). Sophie Wong
  4. I. Rousso et al., "Microsecond atomic force sensing of protein conformational dynamics: Implications for the primary light-induced events in bacteriorhodopsin," PNAS 94, pp. 7937-41 (1997). Matt Gethers, Rosa María Álvarez
  5. F. Schwesinger et al. "Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates" PNAS 97(18): pp. 9972-9977 (2000). Jen Chao

Nov 25: Optical Microscopy: Biomechanics

  1. S. M. Block et al., "Probing the kinesin reaction cycle with a 2D optical force clamp," PNAS 100(5), pp. 2351-56 (2003). Neil Zimmerman, Vivek Thacker
  2. P. J. Verveer et al., "Quantitative Imaging of Lateral ErbB1 Receptor Signal Propagation in the Plasma Membrane," Science 290 pp. 1567-70 (2000). Jessica Keenan
  3. S. Yamada, D. Wirtz, and S. C. Kuo, "Mechanics of Living Cells Measured by Laser Tracking Microrheology," Biophys. J 78(4), pp. 1736-47 (2000).
  4. B. Yap and R. D. Kamm, "Cytoskeletal remodeling and cellular activation during deformation of neutrophils into narrow channels," J Appl. Physiol. 99, pp. 2323-30 (2005).YW
  5. J. C. Crocker et al., "Two-Point Microrheology of Inhomogeneous Soft Materials," Phys. Rev. Lett. 85(4), pp. 888-91 (2000).
  6. C. S. Chen et al., "Geometric control of cell life and death," Science 276 pp. 1425-28 (1997).Jen Logan, Vivian Hernandez
  7. Y. Wang et al., "Visualizing the mechanical activation of Src," Nature 434, pp. 1040-45 (2005).

Dec 9: Optical Trapping and 3D Imaging

  1. Khalil, A.S., et al., "Single M13 bacteriophage tethering and stretching." Proceedings of the National Academy of Sciences 104, pp. 4892-4897 (2007).
  2. Brau, R.R., et al., "Passive and active microrheology with optical tweezers." Journal of Optics A: Pure and Applied Optics 9, pp. S103-S112 (2007).
  3. Y. Nakayama, et al., "Tunable nanowire nonlinear optical probe." Nature 447, pp. 1098-1101 (2007).
  4. JM. Walter, et al., "Light-powering Escherichia coli with proteorhodopsin" Proceedings of the National Academy of Sciences 104, pp. 2408–2412 (2007). Stephanie Nix, Kate B
  5. M. J. Miller et al., "Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node," Science 296 pp. 1869-73 (2002). Luis Somoza
  6. H. Wang et al., "Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissues," Biophys. J 89(1), pp. 581-91 (2005).
  7. K. M. Hanson et al., "Two-Photon Fluorescence Lifetime Imaging of the Skin Stratum Corneum pH Gradient" Biophys. J 83(3) pp. 1682-90 (2002).
  8. P. J. Campagnola et al., "Three-Dimensional High-Resolution Second-Harmonic Generation Imaging of Endogenous Structural Proteins in Biological Tissues," Biophys. J 81(1) pp. 493-508 (2002). Juliana Rotter

PRESENTATION GUIDELINES

Presentation time should be 10 minutes (it's very important that you do not go over this time). We will have 5 minutes for questions and discussion. It's also important that all non-presenters read the papers carefully before the session as this will make the discussion much more interesting.

Your presentation should provide background to motivate why the research was conducted, describe the key results of the paper (not necessarily all of the results) and the essence of the measurement method, and explain the significance of the results to the general field. Remember that 10 minutes will not be nearly enough time to discuss every aspect of the paper so you will need to identify the most important aspects to include in your presentation.

Make sure to upload a Powerpoint or PDF file of your presentation the day before the meeting so that we can use only one computer to avoid connection problems.

Feel free to see 20.309 staff outside of class to discuss any questions or ideas that you might have about the paper.

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