20.309:Schedule

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         of DNA melting lab apparatus
         of DNA melting lab apparatus
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       <p><b>Fri, Sep. 14</b><br>
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       <p><b>Fri, Sep. 14 (RECITATION)</b><br>
         Electronics tutorial
         Electronics tutorial
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       <p><b>Tuesday, Sep. 19</b><br>
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       <p><b>Tue, Sep. 18</b><br>
         Feedback: Black's formula, the loop;<br>
         Feedback: Black's formula, the loop;<br>
         op-amps: &quot;golden rules&quot; and circuit examples </p>
         op-amps: &quot;golden rules&quot; and circuit examples </p>
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       <p><b>Friday, Sep. 22</b></p>
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       <p><b>Thu, Sep. 20</b>
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      Fourier series &amp; integrals; Fourier transform (continuous & discrete)
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      </p>
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      <p><b>Fri, Sep. 21 (RECITATION)</b>
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      MATLAB tutorial and Lab Report Guidelines
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Revision as of 15:38, 28 August 2007

20.309: Biological Instrumentation and Measurement

Home        Course Information        Schedule        People        Student Presentations        LAB SIGNUP       

WEEK LECTURES & RECITATIONS LAB READINGS & ASSIGNMENTS
 
Electronics
0

Thu, Sep. 6
Course Overview & syllabus; Lab Intro & logistics: scheduling, wiki, assignments

Fri, Sep. 7 (LECTURE)
Electronics for DNA analysis; dividers, Thevenin's theorem, impedance and loading, RC circuits

   
1

Tue, Sep. 11
RC circuits: transfer functions, Laplace transforms, impedance, RC filters

Thu, Sep. 13
DNA analysis: SNP detection, chemical equilib. - K and deltaG; description of DNA melting lab apparatus

Fri, Sep. 14 (RECITATION)
Electronics tutorial

Monday, Sep. 10 - Friday Sep. 14
Lab manual for Week 1: Intro to Electronics.
Intro to DC measurements, dividers, impedance; photodiode i-v curves, AC measurements, black-box transfer functions;
the Wheatstone bridge for temperature measurement

Readings
Resistors, capacitors, voltage & current dividers, RC transients.

Impedance, frequency response, filters.

This brief introduction to diodes will be helpful for working on the labs this week.

2

Tue, Sep. 18
Feedback: Black's formula, the loop;
op-amps: "golden rules" and circuit examples

Thu, Sep. 20 Fourier series & integrals; Fourier transform (continuous & discrete)

Fri, Sep. 21 (RECITATION) MATLAB tutorial and Lab Report Guidelines

Monday, Sep. 18 - Friday Sep. 22
Lab manual for Weeks 2 & 3: Measuring DNA Melting Curves.
Build optics for DNA melting experiment, build photodiode readout circuit; calibrate fluorescence signal

Matlab Tutorial file 1 (the basics)

Matlab Tutorial file 2 (curve fitting, etc.)

Readings Operational amplifiers.

 

HOMEWORK SET 1, due Friday, 9/22 by 12:00 noon.

3

Tuesday, Sep. 26
Fourier series, integrals, Fourier transform (continuous/discrete)

Thursday, Sep. 28
Fourier analysis (cont'd)

Thursday, Sep. 28 @ 7:00pm – Room 56-614
Student presentations (Session #1)

NO LAB MONDAY SEP. 25
(Student holiday)

Tuesday, Sep. 26 - Friday, Sep. 29
Lab manual for Weeks 2 & 3: Measuring DNA Melting Curves.
Complete DNA melting curves apparatus; test perfect-match, all-mismatch, and single-base mismatch DNA strands

Readings
Fourier Transforms.

FFT reference material

Thursday, Sep. 28 @ 7:00pm – Room 56-614
Student presentations (Session #1)

 
Mechanics
4

Tuesday, Oct. 3
Scanning probe microscopy (and associated critical band audio files)

Thursday, Oct. 5
Signals, noise, Power Spectral Density

Monday, Oct. 2 - Friday, Oct. 6
AFM alignment and calibration, AFM imaging I

Lab manual for weeks 4-6: The Atomic Force Microscope

Readings
A helpful link that discusses spectral leakage when doing spectral analysis.
See also the FFT reference reading

 

LAB REPORT #1 (Measuring DNA Melting Curves) due Friday, Oct. 6 by 12:00 noon.

5

NO LECTURE TUESDAY, OCT. 10
(Columbus Day)

Thursday, Oct. 12
Correlation/convolution, lock-in amplification, linear systems

NO LAB MON-TUE
(Columbus Day Weekend)

Wednesday, Oct. 11 - Friday, Oct. 13
AFM imaging II; force spectroscopy

Lab manual for weeks 4-6: The Atomic Force Microscope

Readings
Tutorial 1: Overview of Fourier Transforms
Tutorial 2: Sampling and Aliasing

One-page handout, with a few convolution clarification examples.

HOMEWORK SET 2, due Wednesday, 10/11 by 12:00 noon.

6

Tuesday, Oct. 17
Equipartition theorem and thermal fluctuations

Thursday, Oct. 19
Student Presentations (Session #2)

Monday, Oct. 16 - Friday Oct. 20
Thermal fluctuations of microcantilevers: Boltzmann's constant experiment

Lab manual for weeks 4-6: The Atomic Force Microscope

Lab Exercise on Sampling and Aliasing

Thursday, Oct. 19 (in class)
Student Presentations (Session #2)

 
Optics
7

Tuesday, Oct. 24
Image processing I

Thursday, Oct. 26
Image processing II

Supplementary slides to image processing lectures

Monday, Oct. 23 - Friday, Oct. 27
Image processing
Handout on Image Processing

Readings
Great Matlab Matrix Indexing Tutorial

Image Processing Readings (from Gonzalez & Woods):
Chapter 4: histograms, contrast, spatial filtering.
Chapter 8: morphological operations
Chapter 7: segmentation
Chapter 9: recognition & interpretation

8

Tuesday, Oct. 31
Physical optics and optical instrumentation: detectors, noise

Thursday, Nov. 2
Optical instrumentation: sources, lasers

Monday, Oct. 30 - Friday, Nov. 3
Optoelectronics: PMT & photon counting

LAB REPORT #2 (The Atomic Force Microscope) due Monday, Oct. 30 by 12:00 noon.
HOMEWORK SET 3 due (Friday, Nov. 3 by 12:00noon).

9

Tuesday, Nov. 7
Intro to microscopy: geometric optics, lenses, ray tracing

Thursday, Nov. 9
Interference and diffraction, resolution in microscopy, Fourier optics

Monday, Nov. 6 - Friday, Nov. 9
Lab manual for weeks 9-11: Fluorescence microscope construction I: white light imaging and Fourier optics

NO LAB FRIDAY NOV. 10
(Veteran's Day)

Readings
Nice online microscopy references:
"MicroscopyU" from Nikon and Molecular Expressions Optical Microscopy Primer

HOMEWORK SET 3.5 (PMT & photon counting) due Tuesday, Nov. 7 in class.

10

Tuesday, Nov. 14
Fluorescence microscopy

Thursday, Nov. 16
Active microrheology

Monday, Nov. 13 - Friday, Nov. 17
Lab manual for weeks 9-11: Fluorescence microscope construction II: Live-cell imaging & microrheology

 

Thursday, Nov. 16 @ 8:00pm – Room 56-614
Student Presentations (Session #3)

11

Tuesday, Nov. 21
Passive microrheology & Particle tracking

NO LECTURE THURSDAY, NOV. 23
(Thanksgiving)

Monday, Nov. 20 - Wednesday, Nov. 24
Lab manual for weeks 9-11: Fluorescence microscope construction III: Actin cytoskeleton imaging

Papers referenced in the lab manual:
The Vukusic paper on photonic structures
and the Mason and Lau papers on microrheology and the complex viscoelastic modulus.

NO LAB THU-FRI
(Thanksgiving Weekend)

MATLAB scripts for calculating MSD and G*

Three papers about peacock feathers:
Zi, PNAS (2003)
Yoshioka, Forma (2002)
Kinoshita, ChemPhysChem (2005)

12

Tuesday, Nov. 28
Optical trapping

Thursday, Nov. 30
Advanced fluorescence microscopy

Monday, Nov. 27 - Wednesday, Nov. 29
Fluorescence microscope construction/experiments

Wednesday, Nov. 29 - Friday, Dec. 1
Lab manual for weeks 12/13: Optical Trapping

Preprint of AJP paper by Appleyard et al. on the 20.309 traps.

Thursday, Nov. 30 @ 8:00pm – Room 56-614
Student Presentations (Session #4)

13

Tuesday, Dec. 5
3D microscopy: Confocal imaging

Thursday, Dec. 7
3D microscopy: 2-photon microscopy, 3D image processing

Monday, Dec. 4 - Wednesday Dec. 6
Lab manual for weeks 12/13: Optical Trapping

Thursday, Dec. 7 - Friday, Dec. 8
3D Imaging & Visualization: two-photon microscopy

LAB REPORT #3 (Fluorescence Microscope) due Monday, Dec. 4, by 12:00 noon.

14

Tuesday, Dec. 12
Student presentations (Session #5)

Thursday, Dec. 14
End-of-Semester Party

Monday, Dec. 11 - Wednesday, Dec. 13
3D Image-stack Visualization, ImageJ

LAB REPORT #4 (Optical Trapping) due Monday, Dec. 11, by 12:00 noon.

Tuesday, Dec. 12 (in class)
Student presentations (Session #5)

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