3-Aminotriazole

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 7: Line 7:
References:
References:
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
 +
 +
[[Category:Material]] [[Category:Chemical]]

Revision as of 13:30, 19 October 2007

3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Possible carcinogen. Use gloves, work in a hood.

References: Sigma product information

Personal tools